Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
0/8
Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
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Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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 The Cell Structures

The cell is the basic structural and functional unit of all living organisms. It is often referred to as the “building block of life.” Cells can vary in size, shape, and complexity, but they share certain fundamental structures and components.

The major structures commonly found in a typical eukaryotic cell are:

  1. Plasma Membrane: The plasma membrane, also known as the cell membrane, is a thin, flexible barrier that surrounds the cell. It separates the cell’s internal environment from the external environment and regulates the passage of substances in and out of the cell.

  2. Cytoplasm: The cytoplasm is the jelly-like substance that fills the cell between the plasma membrane and the nucleus. It consists of a variety of cellular structures, including organelles, cytosol (the fluid component), and various molecules.

  3. Nucleus: The nucleus is a membrane-bound organelle that houses the cell’s genetic material, DNA (deoxyribonucleic acid). It controls the cell’s activities by regulating gene expression and is responsible for the transmission of genetic information to the next generation.

  4. Mitochondria: Mitochondria are double-membrane-bound organelles that are often referred to as the “powerhouses” of the cell. They generate most of the cell’s energy through the process of cellular respiration, producing ATP (adenosine triphosphate).

  5. Endoplasmic Reticulum (ER): The endoplasmic reticulum is a network of interconnected membranes that extends from the nuclear envelope throughout the cytoplasm. It plays a vital role in the synthesis, folding, and transport of proteins and lipids.

  6. Golgi Apparatus: The Golgi apparatus, or Golgi complex, consists of flattened, membrane-bound sacs called cisternae. It modifies, sorts, and packages proteins and lipids for transport to their final destinations within the cell or for secretion outside the cell.

  7. Lysosomes: Lysosomes are membrane-bound organelles that contain digestive enzymes. They are involved in the breakdown and recycling of cellular waste, cellular debris, and foreign substances.

  8. Peroxisomes: Peroxisomes are small, membrane-bound organelles involved in various metabolic reactions. They play a role in detoxification processes, lipid metabolism, and the breakdown of hydrogen peroxide.

  9. Cytoskeleton: The cytoskeleton is a network of protein filaments that provides structural support, maintains cell shape, and facilitates cell movement. It consists of three main components: microfilaments (actin filaments), microtubules, and intermediate filaments.

  10. Vacuoles: Vacuoles are membrane-bound sacs found in plant and fungal cells, as well as some protists. They have various functions, including storage of water, nutrients, and waste products, as well as playing a role in cell expansion and maintaining turgor pressure in plant cells.

  11. Cell Wall (in plant cells): Plant cells have an additional outer layer called the cell wall, which surrounds the plasma membrane. The cell wall provides structural support, protection, and rigidity to the cell. In addition, plants cells  also have Chloroplast which are the primary organelles for photosynthesis. It contains the pigment chlorophyll

Nonetheless, it’s important to note that prokaryotic cells, such as bacteria, have a simpler structure and lack certain organelles found in eukaryotic cells, such as a nucleus membrane and other membrane-bound organelles.

Difference Between Plant cell and Animal cell

    As shown below, both plant and animal cells share a few common cell organelles, as both are eukaryotes. The function of all these organelles is said to be very much similar. However, the major differences between the plant and animal cells, which significantly reflect the difference in the functions of each cell  are mentioned below:

    Plant cell

     Animal cell

    Cell Shape

    Square or rectangular in shape

    Irregular or round in shape

    Cell Wall

    Present

    Absent

    Plasma/Cell Membrane

    Present

    Present

    Endoplasmic Reticulum

    Present

    Present

    Nucleus

    Present and lies on one side of the cell

    Present and lies in the centre of the cell

    Lysosomes

    Present but are very rare

    Present

    Centrosomes

    Absent

    Present

    Golgi Apparatus

    Present

    Present

    Cytoplasm

    Present

    Present

    Ribosomes

    Present

    Present

    Plastids

    Present

    Absent

    Vacuoles

    Few large or a single, centrally positioned vacuole

    Usually small and numerous

    Cilia

    Absent

    Present in most of the animal cells

    Mitochondria

    Present but fewer in number

    Present and are numerous

    Mode of Nutrition

    Primarily autotrophic

    Heterotrophic

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