Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
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Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
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Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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INTRODUCTION

A cell is defined as the smallest, basic unit of life that is responsible for all of life’s processes. They  are the basic, fundamental unit of life. Cells are the structural, functional, and biological units of all living beings. A cell can replicate itself independently. Hence, they are known as the building blocks of lifeThey  range in size from 0.0001 mm to nearly 150 mm across

                                      The cell structure

All organisms are made up of cells. They may be made up of a single cell (unicellular), or many cells (multicellular).  Mycoplasmas are the smallest known cells. Cells are the building blocks of all living beings.

 Cells are the lowest level of organisation in every life form. From organism to organism, the count of cells may vary. Humans have higher number of cells compared to that of bacteria.

Cells provide structure to the body and convert the nutrients taken from the food into energy.

The study of cells from its basic structure to the functions of every cell organelle is called Cell Biology.

Cells comprise several cell organelles that perform specialized functions to carry out life processes. Every organelle has a specific structure. The hereditary material of the organisms is also present in the cells. Each cell contains a fluid called the cytoplasm, which is enclosed by a membrane. Also present in the cytoplasm are several biomolecules like proteins, nucleic acids and lipids. Moreover, cellular structures called cell organelles are suspended in the cytoplasm.

 Cells are complex and their components perform various functions in an organism. They are of different shapes and size. Our body is made up of cells of different shapes and size

Robert Hooke was the first Biologist who discovered cells in 1665. He observed a piece of bottle cork under a compound microscope and noticed minuscule structures that reminded him of small rooms. Consequently, he named these “rooms” as cells. However, his compound microscope had limited magnification, and hence, he could not see any details in the structure. Owing to this limitation, Hooke concluded that these were non-living entities.

Later Anton Van Leeuwenhoek observed cells under another compound microscope with higher magnification. This time, he had noted that the cells exhibited some form of movement (motility). As a result, Leeuwenhoek concluded that these microscopic entities were “alive.” Eventually, after a host of other observations, these entities were named as animalcules.

In 1883, Robert Brown, a Scottish botanist, provided the very first insights into the cell structure. He was able to describe the nucleus present in the cells of orchids.

The discovery of cells is one of the remarkable advancements in the field of science. It helps us know that all the organisms are made up of cells, and these cells help in carrying out various life processes. The structure and functions of cells helped us to understand life in a better way.

Cell Theory

Cell Theory was proposed by the German scientists,  Theodor Schwann, Matthias Schleiden, and Rudolf Virchow. The cell theory states that:

  1. All living species on Earth are composed of cells.
  2. A cell is the basic unit of life.
  3. All cells arise from pre-existing cells.

modern version of the cell theory was eventually formulated, and it contains the following postulates:

Characteristics of Cells

Following are the various essential characteristics of cells:

  1. Cells provide structure and support to the body of an organism.
  2. The cell interior is organised into different individual organelles surrounded by a separate membrane.
  3. The nucleus (major organelle) holds genetic information necessary for reproduction and cell growth.
  4. Every cell has one nucleus and membrane-bound organelles in the cytoplasm.
  5. Mitochondria, a double membrane-bound organelle is mainly responsible for the energy transactions vital for the survival of the cell.
  6. Lysosomes digest unwanted materials in the cell.
  7. Endoplasmic reticulum plays a significant role in the internal organisation of the cell by synthesizing selective molecules and processing, directing and sorting them to their appropriate locations.

Types of Cells

Cells are similar to factories with different labourers and departments that work towards a common objective. Various types of cells perform different functions. Based on cellular structure, there are two types of cells:

  1. Prokaryotes
  2. Eukaryotes

Prokaryotic Cells

  1. Prokaryotic cells have no nucleus. Instead, some prokaryotes such as bacteria have a region within the cell where the genetic material is freely suspended. This region is called the nucleoid.
  2. They all are single-celled microorganisms. Examples include archaea, bacteria, and cyanobacteria.
  3. The cell size ranges from 0.1 to 0.5 µm in diameter.
  4. The hereditary material can either be DNA or RNA.
  5. Prokaryotes generally reproduce by binary fission, a form of asexual reproduction. They are also known to use conjugation – which is often seen as the prokaryotic equivalent to sexual reproduction (however, it is NOT sexual reproduction).

Eukaryotic Cells

  1. Eukaryotic cells are characterized by a true nucleus.
  2. The size of the cells ranges between 10–100 µm in diameter.
  3. This broad category involves plants, fungi, protozoans, and animals.
  4. The plasma membrane is responsible for monitoring the transport of nutrients and electrolytes in and out of the cells. It is also responsible for cell to cell communication.
  5. They reproduce sexually as well as asexually.
  6. There are some contrasting features between plant and animal cells. For eg., the plant cellcontains chloroplast, central vacuoles, and other plastids, whereas the animal cells do not
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