Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
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Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
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Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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INTRODUCTION

Although life at the macro level is diverse, the chemistry making up that life is remarkably similar. All living things are made up of basic building blocks called elements. An element is a substance that cannot be broken down into simpler substances using chemical means. Carbon, oxygen, hydrogen, nitrogen, sulfur, calcium, sodium and iron are examples of elements you will come across in Life Sciences.

Each element is distinguished by the composition of its atom. An atom is the basic unit of matter. Molecules are formed when one or more atoms are covalently bonded together. The atoms of a molecule can be identical, such as O2 or H2 or differ such as H2O. A compound is formed when atoms of different elements join together. Because all compounds contain more than one atom, all compounds are molecules. However, not all molecules are compounds.

Compounds are divided into organic and inorganic compounds.  Organic compounds always contain carbon, but not all compounds that contain carbon are organic. A general rule of thumb is that organic compounds contain carbon, with at least one of these carbons bonded to hydrogen atoms.

 Carbon dioxide is therefore an inorganic compound even though it contains carbon.

The major organic compounds found in living organisms include: carbohydratesfatsproteins and nucleic acids.

Inorganic compounds 

  1. The role of water in the maintenance of life 
  2. As shown in the table above, up to 65% of our bodies are made up of water. Water is an inorganic compound made up of two hydrogen atoms and one oxygen atom. Its molecular formula is H2 Water plays an important role in the maintenance of biological systems.
  3. Temperature regulation: in humans, the sweat glands produce sweat which cools the body as it evaporates from the body surface in a process called perspiration. In a similar way, plants are cooled by the loss of water vapour from their leaves, in a process called transpiration.
  4. Form and support: water is an important constituent of the body and plays an important role in providing form and support in animals and plants. Animals, such as worms and jellyfish, use water in special chambers in their body to give their bodies support. This use of water pressure to provide body form, and enable movement is called a hydrostatic skeleton . Plants grow upright and keep their shape due to the pressure of water (turgor pressure) inside the cells.
  5. Transport medium: water transports substances around the body. For example, water is the main constituent of blood and enables blood cells, hormones and dissolved gases, electrolytes and nutrients to be transported around the body.
  6. Lubricating agent: water is the main constituent of saliva which helps chewing and swallowing and also allows food to pass easily along the alimentary canal. Water is also the main constituent of tears which help keep the eyes lubricated.
  7. Solvent for biological chemicals: the liquid in which substances dissolve is called a solvent. Water is known as the universal solvent as more substances dissolve in water than in any other liquid.
  8. Medium in which chemical reactions occur: all chemical reactions in living organisms take place in water.
  9. Reactant: water takes place in several classes of chemical reactions. During hydrolysis reactions, water is added to the reaction to break down large molecules into smaller molecules. Water can also be split into hydrogen and oxygen atoms to provide energy for complex chemical reactions such as photosynthesis.

Minerals 

Dietary minerals are the chemical elements that living organisms require to maintain health. In humans, essential minerals include calcium, phosphorous, potassium, sulfur, sodium, chlorine and magnesium.

Macro-elements (macro-nutrients) are nutrients that are required in large quantities by living organisms (e.g carbon, hydrogen, oxygen, nitrogen, potassium, sodium, calcium, chloride, magnesium, phosphorus and sulfur).

Micro-elements (micro-nutrients) are nutrients that are required in very small quantities for development and growth and include iron, cobalt, chromium, copper, iodine, manganese, selenium, zinc and molybdenum.

Nutrients required for human health

The tabble below summarises some important minerals required for proper functioning of the human body. Proper nutrition involves a diet in which the daily requirements of the listed mineral nutrients are met.

Mineral

Food Source

Main Functions

Deficiency Disease

Macro-nutrients

Calcium (Ca)

most fruit and vegetables, meat, dairy products

strong bones and teeth; muscle contraction; blood clotting; nerve function

rickets, osteoporosis

Magnesium (Mg)

nuts, meat, dairy products

strong bones and teeth; nerve and muscle function; energy production

osteoporosis, muscle cramps

Phosphorus (P)

nuts, meat, dairy products

strong bones and teeth; nerve function; part of nucleic acids and cell membranes

rickets, osteoporosis

Potassium (K)

bananas, meat, dairy products

growth and maintenance, water balance, heart function

muscle cramps; heart, kidney and lung failure

Sodium (Na)

table salt, fruit and vegetables

regulates blood pressure and volume; muscle and nerve function

muscle cramps

Sulfur (S)

meat, dairy products, eggs, legumes

part of proteins; detoxifies the body; good skin; hair and nails

disorder unlikely

Micro-nutrients

Iron (Fe)

meat, legumes

part of haemoglobin (the oxygen transport protein); part of some enzymes

anaemia

Iodine (I)

seafood, iodated salt

production of hormones by the thyroid gland; strong bones and teeth; good hair; skin and nails

goitre, stunted growth, mental problems

Zinc (Zn)

seafood, meat

immune function; male reproductive system

stunted growth, prostate problems

Minerals required by humans.

Nutrients required for plant growth

The previous section examined the key nutrients important for animal growth. In Table 1.4 we will now look at the key nutrients required for plant growth.

 Chlorosis is the yellowing of the leaves due to low production or loss of chlorophyl

Mineral

Source

Main Functions

Deficiency Disease

Macro-nutrients

Calcium (Ca)

inorganic fertilizers; Ca ions in the soil

part of the plant cell wall; transport and rentention of other elements

chlorosis (yellowing of the leaves due to low production or loss of chlorophyll)

Magnesium (Mg)

inorganic fertilizers; Mg ions in the soil

component of chlorophyll (pigment for photosynthesis); activates many enzymes required for growth

chlorosis

Nitrogen (N)

inorganic fertilizers in the form of nitrates; symbiotic nitrogen-fixing bacteria in roots

component of chlorophyll; nucleic acids and proteins; seed and fruit production

stunted growth; smaller leaves

Phosphorus (P)

inorganic fertilizers in the form of phosphates; low amounts in the soil

photosynthetic process; part of nucleic acids and cell membranes; root growth

stunted growth, blue/green leaves

Potassium

inorganic fertilizers; K ions in the soil

needed for protein synthesis, photosynthesis, enzyme activation, opening and closing of stomata;

chlorosis; curling leaf tips; brown scorching, poor fruit quality

Sulfur (S)

inorganic fertilizers

protein synthesis; root growth; chlorophyll formation; promotes activity of enzymes

chlorosis

Micro-nutrients

Iron (Fe)

inorganic fertilizers; Fe ions in the soil

component of the enzyme that makes chlorophyll

chlorosis

Zinc (Zn)

inorganic fertilizers; Zn ions in the soil

part of growth-regulating enzyme systems

poor leaf growth

Sodium (Na)

inorganic fertilizers; Na ions in the soil

maintains salt and water balance

reduced growth

Iodine (I)

inorganic fertilizers; I ions in the soil

needed for energy release during respiration

poor growth

 

Nutrients required for plant growth.

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