Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
0/8
Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
0/5
Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
0/4
Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
0/4
Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
0/12
Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
0/9
Biology Techniques For Science Laboratory Technicians
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Lymphatic circulatory system

The lymphatic system is part of the circulatory system, comprising a network of inter-connected tubes known as lymphatic vessels that carry a clear fluid called lymph towards the heart.

The lymphatic organs play an important part in the immune system. The lymphatic system transports the white blood cells which are important in the immune response against pathogens.

Composition of Lymphatic system

The lymphatic system is composed of lymph vessels, lymph ducts, lymph nodes and organs. The organs associated with the lymphatic system are the spleen and thymus. The spleen is the boundary between the blood and the lymphatic system. Knots of lymphatic tissue in the spleen add lymphocytes to the blood. The spleen also acts as a filter for the blood, and helps to destroy worn out red-blood cells. In the event of damage to the spleen, it can be removed and its functions will be carried out by the liver, the bone marrow and the lymph nodes.Lymph vessels are located as a network throughout all tissues in the body. Lymph vessels assist the circulatory system and all the cells of the body by removing wastes, germs and excess water from the tissue fluid. Lymph vessels carry lymph fluid from the bottom of the body up towards the heart and also drains from the head and shoulders as well as the arms.

Muscle contractions help push the lymph fluid upwards, and valves prevent the lymph fluid from flowing backwards. Many lymph vessels eventually merge into two large lymphatic vessels, called lymphatic ducts which empty into veins in the neck. The thoracic duct collects from the left side of the body and the lower right side of the body and empties into the left subclavian vein. The right thoracic duct collects from the right arm, thorax, neck and head, and drains into the right subclavian vein.

                     Diagram of human lymphatic system.

Most of the disease-fighting function of the adult mammal is carried out by the lymph nodes which occur along the lymph ducts. Lymph nodes are small, irregularly-shaped masses through which lymph vessels flow. Clusters of nodes occur in the armpits, groin, and neck. Cells of the immune system line channels through the nodes and attack bacteria and viruses travelling in the lymph, so they basically act as tiny filters.

Interaction between lymphatic and cardiovascular circulatory systems.





Functions of lymphatic system 

The main functions of the lymphatic system are as follows:

The main function of the lymphatic system is to collect and transport tissue fluids from the intercellular spaces in all the tissues of the body, back to the veins in the blood system.

Lymph plays an important role in returning plasma proteins to the bloodstream.

Digested fats are absorbed and then transported from the villi in the small intestine to the bloodstream via the lymph vessels.

Lymphocytes are manufactured in the lymph nodes Antibodies manufactured in the lymph nodes assist the body to build up an

effectiveimmunity to infectious diseases.

Lymph nodes play an important role in the defence mechanism of the body. They filter out micro-organisms (such as bacteria) and foreign substances such as toxins.

Lymph transports large molecular compounds (such as enzymes and hormones) from their manufactured sites to the bloodstream.           Elephantiasis is a disease characterised by thickening of the skin and underlying tissues, especially in the legs and genitals. It occurs when the body becomes infected by parasitic infections, which target the lymphatic system

.A comparison of the cardiovascular and lymphatic systems.

Cardiovascular System

Lymphatic System

Blood is responsible for collecting and distributing oxygen, nutrients and hormones to the tissues of entire body.

Lymph is responsible for collecting and removing waste products left behind in the tissues.

Blood flows in the arteries, capillaries, and veins.

Lymph flows in an open circuit from the tissues into lymphatic vessels.

Blood flows towards the heart and away from the heart.

Lymph flows in one direction only (towards the heart).

Blood is pumped by the heart to all parts of the body.

Lymph is not pumped. It passively flows from the tissues into the lymph capillaries.

Blood consists of the liquid plasma that transports the red and white blood cells and platelets.

Lymph that has been filtered and is ready to return to the cardiovascular system is a clear or milky white fluid.

Blood is visible and damage to blood vessels causes obvious signs such as bleeding or bruising.

Lymph is colourless or translucent and damage to the lymphatic system is difficult to detect until swelling occurs.

Blood is filtered by the kidneys.

Lymph is filtered by lymph nodes located throughout the body.


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