Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
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Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
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Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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Microscopes

Microscopes are powerful tools used to magnify and visualize objects that are too small to be seen with the naked eye. They enable scientists, researchers, and professionals in various fields to observe and study microscopic details of samples and specimens.

Microscopy is the scientific field concerned with the study and use of microscopes.

There are several types of microscopes, each with its own specific principles and applications. The most common types of microscopes include:

  1. Optical Microscopes: Optical microscopes use visible light and a series of lenses to magnify and resolve the details of a sample. They are versatile and widely used in many scientific disciplines. Optical microscopes include compound microscopes, which use multiple lenses to magnify the image, and stereo microscopes, which provide a three-dimensional view of the specimen.

  2. Electron Microscopes: Electron microscopes use a beam of electrons instead of light to visualize samples. They have much higher magnification and resolution capabilities compared to optical microscopes. Two main types of electron microscopes are transmission electron microscopes (TEM), which transmit electrons through a thin specimen, and scanning electron microscopes (SEM), which scan the surface of the specimen with a focused electron beam.

  3. Scanning Probe Microscopes: Scanning probe microscopes (SPMs) work by scanning a tiny probe over the surface of a sample, measuring interactions between the probe and the surface to create an image. Different types of SPMs include atomic force microscopes (AFM) and scanning tunneling microscopes (STM). These microscopes provide extremely high-resolution imaging and can even measure properties like atomic forces and electrical conductivity.

Microscopy plays a crucial role in numerous scientific disciplines and applications, including:

  1. Biology and Medicine: Microscopes are essential in biological research and medical diagnostics. They allow scientists and healthcare professionals to study cells, tissues, microorganisms, and other biological structures in detail. Microscopy techniques like fluorescence microscopy, confocal microscopy, and immunohistochemistry help visualize specific molecules and markers within biological samples.

  2. Materials Science: Microscopy is used in materials science to investigate the structure, composition, and properties of materials at the micro and nano scale. It helps researchers understand the morphology, crystal structure, defects, and surface properties of various materials, including metals, ceramics, polymers, and semiconductors.

  3. Forensics: Microscopy is employed in forensic science for the examination and analysis of trace evidence, such as fibers, hairs, fingerprints, and tool marks. Microscopic analysis can provide valuable information in criminal investigations and court proceedings.

  4. Nanotechnology: Microscopes, especially electron microscopes and scanning probe microscopes, are vital in nanotechnology for visualizing and manipulating nanostructures and nanoparticles. They enable researchers to study and engineer materials and devices at the atomic and molecular scale.

  5. Environmental Science: Microscopy is used in environmental science to analyze and identify microscopic organisms, such as algae, bacteria, and fungi, in water and soil samples. It helps monitor environmental health, assess pollution levels, and study ecosystems.

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