Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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Introduction to Microscopes and Microscopy Techniques
Types of Microscopes
The Optical(Light) Microscope
Magnifications and Resolving Power of Microscopes
Use and Care of Microscopes
The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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INTRODUCTION
The Cell Structure
The Cell Functions
Cell Division
The Cell Cycle
Mitosis
Meiosis
Similarities and Differences between Mitosis and Meiosis
Microscope Slides
Microscope Slides Preparartion
Tissue Preparation For Microscpy
Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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INTRODUCTION
Classification of Microorganisms
Bacteria
Classification of Bacteria
Nutrition in Bacteria
Reproduction in Bacteria
Economic Importance of Bacteria
Fungi
Classification of Fungi
Nutrition in Fungi
Reproduction In Fungi
Economic Importance of Fungi
Virus
Classification of Virus
Reproduction in Virus
Economic Importance of Virus
Culture Media
Preparation of Culture Media
Culture Methods
Culture Techniques
Microbiological Stains and Staining Techniques
Microbiological Staining Techniques
Sterilization
Chemicals of Life
THE CHEMICALS OF LIFE OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain meaning of terms. 2.Describe chemical substances found in living organisms. 3.Describe the functions of substances found in living organisms. 4.Describe enzymes. 5.Carry out food tests.
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INTRODUCTION
Carbohydrates
Structural composition of Carbohydrates
Chemical Tests for Carbohydrates
Lipids
Structural composition of Lipids
Saturated and Unsaturated fats
Tests for Lipids
Proteins
Structural composition of Proteins
Protein Denaturation
Properties of Proteins
Test for Proteins
Nucleic Acids
Vitamins
Enzymes
Classification of Enzymes
Mechanisms of Enzyme Action
Factors Affecting Enzyme Activities
Enzyme Inhibition
Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
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INTRODUCTION
Types of Immunity
Lymphocytes and Lymphatic System
The White Blood Cells  (Leucocytes )
Lymphoid Organs ,Tissues and Immune Cells
Immunoglobulins
Antigens
Immunological Techniques
Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Introduction
Collection of Plant Specimens
Collecting techniques
Preservation Techniques
Processing of Specimens
Storage of Specimens
Pests In The Herbarium
Green House and Botanical Garden
Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
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INTRODUCTION
Collection of Museum Specimens
Preservation of Museum Specimens
Preservation of other animals specimens
Labeling of museum specimens
Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Introduction
Setting Up A vivarium
Ecological Requirements
Care and Handling
Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Introduction
Setting up an Aquarium
Basic Physical and Ecological requirements
Feeding and General care of Aquarium
Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction
General Care of Laboratory Animals
Laboratory Animal House
Feeding of Laboratory Animals
Disease and Disease control in Laboratory animals
Sexing of Laboratory Animals
Identification of Laboratory Animals
Dosing of Laboratory animals
Drawing blood from Lab animals
Administering anesthesia to laboratory animals
Humane Killing of Laboratory Animals
Dissecting Laboratory Animals
Conventional and Specific Pathogen free animals
Gnotobiotic Animals
Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Introduction
The Biosphere and Biomes
The Ecosystem
Energy Flow
Estimation of Population size
Nutrient Flow in Ecosystem
Water cycle
Carbon Cycle
Nitrogen Cycle
Sulfur Cycle
Phosphorous Cycle
Ecological succession
Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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INTRODUCTION
Plant Vascular Tissues
Plant Leaf Structure
Transport of substances into and out of the leaf 
Nutrition in Plants
Transpiration in Plants
Translocation in plants
Reproduction in Plants
Excretion in Plants
Animal Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of animal parts and tissues. 2.Describe functions of various animal tissues and organs. 3.Describe physiological processes in animals .
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Nutrition In Animals
Types of Nutrition  in Animals
Digestion in Humans
Digestion in Ruminants
Respiration in Animals
Modes of Gaseous Exchange in Animals
Mammalian Respiratory System
Excretion in Animals
Excretion in Higher Animals
Excretory System in Man:
Transport In Animals
Circulatory systems in animals
Human circulatory systems 
The cardiac cycle 
The Blood Vessels
Lymphatic circulatory system
Cardiovascular diseases 
Support and Locomotion system in Animals
Exoskeleton and Endoskeleton
Human Skeleton
The Bones 
Musculoskeletal tissues 
The Nervous System
The Nerve Tissue
Organization of the Nervous System
Reproductive system in Animals
Male Reproductive System
Female Reproductive System
The Endocrine System
Endocrine Glands & Their Hormones
Biology Techniques For Science Laboratory Technicians
About Lesson

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Magnification and Resolving power of a light microscope

Magnification is simply the ability to make tiny things appear big. Increase in magnifying power  is achieved by using a wide range of  objective lens . Magnifying power is always linked to  the resolving power .  The higher the magnification , the higher the resolving power and hence the closer the details of the specimen  can be seen .

the resolving power of an objective lens depends on the numerical aperture (NA) of that objective lens. The following are the usual NA values of the commonly used objective lens

X10 objective lens = NA 0. 25  

X20 objective lens  = NA 0.45

X40 objective lens  = NA 0.65

X100 objective lens = NA 1.25

Immersion Oil

When a beam of light passes from Air          Glass           Air        Glass,   It’s normally bent due to refraction property of light  . These bending have little effect on low power objective lens  i.e. X10, X20, X40 objectives , but it have significant limitation  to the amount of light  which enter through the high power objective lens i.e.  X100 objective lens and consequently its resolving power.

Such bedding can be avoided by replacing the air between the specimen and the lens with an oil Immersion which have the same optical property as that of glass. These makes light to pass in a straight line as though it was passing through the same media i.e. glass all the way. These therefore provides better resolving power.

Chromatic and spherical aberration

Chromatic aberration occurs when a biconcave lens splits whit light into its component colors and in the process blue light is magnified more than red light so that blue comes into focus near to the lens. While spherical aberration is due to the edges of the lens giving slightly higher magnification than its center.

 Illumination system of the light microscopes  

Good microscopy requires an adequate well-ventilated and controllable illumination system. These can be achieved by using a microscope with an inbuilt illumination. Daylight illumination should be discouraged because its variable, difficult to use and rarely adequate for oil emulsion work.

Glare  

Glare in microscopes is simply that inconvenience caused as a result of light reaching the eye  which does not go to making  up the perfect image  but instead only interferes with the image and the ability of the objective to  distinguish details in a specimen   . To test for glare, remove the eyepiece and check  to see if the inside of the tube is illuminated . These indicate presence of glare. Glare can be reduced in the following ways

  • Positioning a microscope with an inbuilt illumination in a subdued light i.e. not in front of a window
  • Avoid using a large source of illumination than its necessary
  • Reducing the condenser glare by reducing the condenser aperture i.e. by adjusting the iris diaphragm when using low power objective

Microscope filters

 Filters are special devices in microscopes  used to;

  • Reduce the intensity of light when it is required
  • To increase contrast and resolution
  • To transmit light of selected wavelength
  • To protect the eyes from injuries caused by UV light
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