Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
0/8
Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
0/8
Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
0/5
Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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Saturated and unsaturated fats

Carbon can form four bonds with other atoms. Most carbons in a fatty acid chain are bonded to two adjacent carbons, and to two hydrogen atoms. When each carbon atom in a fatty acid chain forms four single bonds and has the maximum number of hydrogen atoms, the fatty acid chain is called saturated because it is “saturated” with hydrogen atoms. However, sometimes two adjacent carbons will from a double bond. In this case the carbons taking part in the double bond are each joined to only one hydrogen. Fatty acids that have carbon-carbon double bonds are known as unsaturated, because the double bond can be ‘broken’ and an additional bond with hydrogen can be made. Double bonds are stronger than single bonds and they give the fatty acid chain a ‘kink’. These kinks mean that the molecules can not pack together tightly, and the lipids are more fluid. This is why unsaturated fats tend to be liquid at room temperature, while saturated fats tend to be solid. Fatty acid chains with many double bonds are called poly-unsaturated fatty acids.

Cholesterol

Cholesterol is an organic chemical substance known as a sterol.  It is an important component in cell membranes. The major dietary sources of cholesterol include cheese, egg, pork, poultry, fish and shrimp. Cholesterol is carried through the body by proteins in the blood known as lipoproteins. A lipoprotein is any combination of lipid and protein.

Cholesterol is carried in the blood through the body by high density lipoproteinlow density lipoprotein and through triglycerides.

  1. Low density lipoprotein (LDL): Low density lipoprotein transports cholesterol around the body. It has a higher proportion of cholesterol relative to protein. It is often known as “bad” cholesterol because higher levels of LDL are associated with heart disease.
  2. High density lipoprotein (HDL): High density lipoprotein is the smallest of the lipoproteins. It has a high proportion of protein relative to cholesterol and is therefore often known as the “good” cholesterol. HDL transports cholesterol away from cells and to the liver where it is broken down or removed from the body as waste.

High levels of LDL can cause heart disease. Cholesterol builds up in blood vessels that carry blood from the heart to the tissues and organs of the body, called arteries. This leads to a hardening and narrowing of these vessels, which interferes with the transport of blood, and can potentially lead to a heart attack. The biggest contributor to the amount of cholesterol in your blood is the type of fats you eat. Saturated fats are less healthy than unsaturated fats as they increase the amount of LDL cholesterol in your blood.

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