Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
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Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
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Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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RESPIRATION IN ANIMALS

Respiration is the process of releasing energy from food and this takes place inside the cells of the body. The process of respiration involves taking in oxygen (of air) into cells, using it for releasing energy by burning food, and then eliminating the waste products (carbon dioxide and water) from the body. Respiration is essential for life because it provides energy for carrying out all the life processes which are necessary to keep the organisms alive.

Respiration is the process of releasing energy from food and this takes place inside the cells of the body. The process of respiration involves taking in oxygen (of air) into cells, using it for releasing energy by burning food, and then eliminating the waste products (carbon dioxide and water) from the body. Respiration is an essential for life because it provides energy for carrying out all the life processes which are necessary to keep the organisms alive.

Breathing and Respiration:

The mechanism by which organisms obtain oxygen from the air and release carbon dioxide is called breathing. But on the other hand respiration is a complex process it includes breathing as well as the oxidation of food in the cells of the organism to release energy. Breathing is a physical process whereas respiration is a biochemical process of oxidation of food. Breathing process involves lungs of the organism whereas respiration involves the mitochondria in the cells where food is oxidised to release energy.

Procedure of how energy is released during respiration:

The energy produced during respiration is stored in the form of ATP (Adenosine Tri- Phosphate) molecules in the cells of the body and used by the organism as when required. To understand this we should know the meaning of ADP, ATP and Inorganic Phosphate. ADP is Adenosine Di- Phosphate; ATP is Adenosine Tri- Phosphate and Inorganic Phosphate is a substance which contains a phosphate group made up of phosphorous and Oxygen.

(i) The energy released during respiration is used to make ATP molecules from ADP and inorganic phosphate.

This energy is stored in the cells in the form of ATP.

(ii) When the cells need energy, then ATP can be broken down using water to release energy.

The released energy by ATP is equivalent to 30.5 kJ/mole and is used to carry out all the endodermic reactions taking place in the cells and so, ATP is known as the currency of cells.

Types of Respiration:

  • Aerobic Respiration:The respiration which uses oxygen. In this process, the glucose food is completely broken down in to carbon dioxide and water by oxidation and this energy gets stored in the ATP molecules.

Thus, mitochondria are the sites of aerobic respiration in the cells.

  • Anaerobic Respiration:The respiration which takes place without oxygen. In this, the microorganisms like yeast break down glucose (food) in to ethanol, carbon dioxide and releases energy.

Therefore, whole process of anaerobic respiration takes place in the cytoplasm of cells. So, we can say that all the cells do not use oxygen to produce energy. Fermentation of sugar can also be done by using anaerobic respiration of yeast.

Human beings obtain energy by aerobic respiration but sometimes anaerobic respiration takes place in our muscles during vigorous physical exercise when oxygen gets used up faster in the muscle cells than can be supplied by the human body.

The accumulation of lactic Acid in the muscles causes muscle cramps.

  • There is one similarity between aerobic and anaerobic respiration and that is energy is produced by the breakdown of food like glucose. The main differences between them  are explained below:

Aerobic Respiration

Anaerobic Respiration

1. It takes place in the presence of oxygen.

1. It takes place in the absence of oxygen.

2. Complete breakdown of food occurs.

2. Partial breakdown of food occurs.

3. The end products in aerobic respiration are carbon dioxide and water.

3. The end products in anaerobic respiration may be ethanol and carbon dioxide (as in yeast plant) or lactic acid (as in animal muscles).

4. In this considerable amount of energy is produced.

4. Much less energy is produced.

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