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Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
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Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
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Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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Lymphoid Organs ,Tissues and Immune Cells

Immune system consist of interconnecting  network  of organs and tissues . The dominant cells in these  organs and tissues are lymphocytes  and therefore these organs and tissues are known as lymphoid .

The immune system consist of a number of  lymphoid organs  including

  • Thymus glands
  • Lymph nodes
  • Spleen
  • Tonsils
  • Aggregates of lymphoid tissues  in noon lymphoid organs e.g. payers patches in the gut
  • Bone marrow tissues in the long bones

The thymus and the bone marrow are referred to as the central  or primary lymphoid  organs or system  while the rest are referred to as  peripheral or secondary  lymphoid organs . These is because development  and function of the  peripheral lymphoid organs  depends absolutely  on  the  cells made  by the thymus and  the bone marrow  

The Primary (Central ) Lymphoid Organs

  1. Thymus Glands

Thymus gland is a lobed  organ located  in the thoracic cavity  just above the heart. It consists of many lobes each of which contains a cortex and medullar.

Thymus gland begins to function in the embryonic stage   but its at its most active stage at time near or shortly after birth.

It however decreases in size after weaning period  and will soon stop functioning . Thymus gland is important in the development of immune response  in an individual . removal of thymus is called thymectomy . absence of thymus gland  in new born leads to premature death due to lack of lymphocytes  in the blood and tissue fluids  (lymphopenia ) the animal may also lack ability to  recognize and react to tissue transplant  (does not reject allografts ) but these however is not the case in older  animals  these may be due to the reason that the function of the thymus glands  are carried out earlier in the animals life  and hence the thymus becomes less important  so long as the remaining parts of the immune system are functional .

The thymus glands functions by  accepting the stem cells from the bone marrow  at the time of birth  and ensures completion of the development . They increase in sizes  and develop to mature T – lymphocytes  (thymocytes ) . These thymocytes package themselves in the  medullar and cortex of the thymus  glands . Some of the  thymocytes  mature  into different classes  of mature T-cells  e.g. helper  T-cells , suppressor T-cells  and killer T –cells .  Others leave  the thymus gland  through the blood stream  and migrates into the lymph nodes , spleen , tonsils , payers patches  etc. where they form   mature T-cells

T-cells are involve both in the cell mediated  and humoral immune system . during the process of  maturation , the T-cells  becomes immunocompetent  i.e. they gain the ability to  respond  to specific antigens , they also gain the ability to distinguish  between self and non self antigens  in the body . each T-cells produces small  proteins  molecules  called lymphokynes  (or cytokynes or  interleukins ). Lymphokynes  serve to  make T- cells keep on  multiplying , promote inflammation  and  stimulates B-cells  to start making antibodies

  1. Bursa of Fabricious  

These is another  primary lymphoid organ  found in birds  in addition to thymus. It is situated near the cloaca . Stem cells enter  the bursa of fabricous where they differentiate into B-cells  that are capable of producing  antibodies.Removal of bursa of fabricous is known a bursectomy. Bursectomised a birds lack B-cells  and therefore cannot produce antibodies but they  are able to reject graft .

Mammals including man  have no bursa of fabricous  yet they have B –cells These means  that in mammals  the bone marrow serves an equivalent role  as the bursa of fabricous

  1. The Bone Marrow

Erythrocytes , leucocyte  and platelets  are produced in the bone marrow  by a process called  hemoporesis . The stem cells that give rise to these blood cells are called  hemopoetic  stem cells .

Precursors of the lymphocytes originates in the bone marrow but  they latter migrate  in the blood stream to the thymus  where they differentiate into  T-  lymphocytes.

When these stem cells  migrate  to the spleen  or lymphnodes  or remain in the bone marrow , they differentiate into B-lymphocytes . The formation of lymphoid outside the bone marrow  is known as  lymphoporesis .

The blood cells formation depends on the presence of hemopoetic stem cells  in the bone marrow (precursor cells)

Earlier in the 19th and 20th century, blood cells  were  put into two categories  according to their presumed origin  i.e.

  • Erythrocytes , granular  leukocytes  and platelets  developing from the bone marrow were called  myeloid  and their formation  was referred to as myelopoesis .
  • Lymphocytes and monocytes  were believed to originate in the lymph nodes , spleen  and thymus . They were hence referred to as lymphoid elements  of blood and therefore there formation  was called lymphopoeisis.

But however recent studies have shown that  lymphopoetic stem cells  similarly originates from the bone marrow

Secondary Lymphoid Organs

  • Lymph nodes

The net work of the lymph vessels in the body forms  the lymphatic system . At some points in the lymphatic system ,are some swellings referred to as lymph nodes . Lymph nodes are located in some strategic places  in the body such as in the armpits ,neck regions  and groins  etc . Each lymph node  receives   a flow of lymph  through its afferent lymph vessels.

Each lymph node is a spherical or bean shaped  and it is surrounded by a tough  membrane  or capsule . Beneath these capsule is  a network of phagocyte lined mesh work  called subcapsular sinus . These meshwork region  filters any microorganisms present and phagocytes or engulfs  them . phagocytes present in these region are macrophages .

The lymph nodes have a densely packed  lymphocytes and macrophages  organized into spherical nests  or cells called follicles. When stimulated by an antigen , these follicles becomes enlarged  and the cells inside multiply rapidly  particularly at the center  (germinal center )

An active follicle produces lymphocytes and  plasma cells  which in turn produces  antibodies . The antibodies are produced as a result of presence or introduction  of any antigen . These explains why armpits  lymph nodes  due to a wound on a  finger .the wound allows entrance of antigens  into the body  which find their way into the  lymph node were they are trapped . In the lymph nodes the follicles becomes very active by enlarging and producing many lymphocytes  and macrophages . The lymphocytes differentiates into  plasma cells  which then make antibodies  which shall fight against the antigens introduced . The macrophages engulf the bacteria  at faster rate .all  these  activities  makes  the lymph nodes to swell  and become painful

  • Spleen

The spleen has two major functions ;

  • It destroys old red blood cells
  • It’s a major site for immune response

the spleen function just like the lymph node  but the main difference is that  the fluid it deals with is blood and not lymph .The spleen have two major parts  (I) the red pulp  and (ii)  the white pulp

The red pulp deals with destruction of  red blood cells  while the white pulp  is where the immune response  are generated . The lymphocytes are concentrated in the white pulp  which surrounds the central artery called  periarterial lymph node .

The T- lymphocytes are found in the  central  parts  of the pulp  while B-lymphocytes  are found  in the germinal centers  of the lymph follicles . The white pulp is separated  from the red pulp by  a marginal zone . The lymphocytes enter  and leave the  pulp via  the capillaries  of the central arteries  in he marginal zone . Some mature plasma cells pass  from  the marginal zone  to the red pulp .

The marginal zone is where the blood lymphocytes ,antigens and tissue fluids are deposited , there also intense  phagocytic activities  carried out by macrophages

Other lymphoid tissues

  1. Gut associated lymphoid  tissues (GALT)g. payers patches  and appendix .

These tissues contain both  T and B  independent  areas  which contain T and B lymphocytes

  1. Mucosa Associated Lymphocytes  Tissues (MALT)

They are found in the gut, urinary tract and respiratory tract . they have both T and B lymphocytes

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