Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
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Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
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Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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Structural composition of Carbohydrates

Carbohydrates are organic compounds composed of carbon, hydrogen, and oxygen atoms. 

Carbohydrates are made up of monomers known as monosaccharides  which is a single sugar molecule .Monosaccharides  form basic structural unit of carbohydrates of  all carbohydrates , .The monosaccharide that makes up most carbohydrates is glucose. Other monosaccharides include .

  • Glucose: Glucose is a hexose monosaccharide and the most important sugar in biology. It is a primary source of energy for cells and is commonly referred to as blood sugar. Glucose is found in various foods like fruits, honey, and starchy vegetables.

  • Fructose: Fructose is another hexose monosaccharide and is commonly known as fruit sugar. It is naturally present in fruits, vegetables, and honey. Fructose is sweeter than glucose and is often used as a sweetener in processed foods and beverages.

  • Galactose: Galactose is a hexose monosaccharide that is less common than glucose and fructose. It is found in dairy products, certain fruits and vegetables, and is a component of lactose (milk sugar). In the body, galactose is converted into glucose for energy.

  • Ribose: Ribose is a pentose monosaccharide that is an important component of nucleotides, the building blocks of RNA (ribonucleic acid). It is involved in the synthesis of genetic material and is found in all living cells.

  • Deoxyribose: Deoxyribose is a modified form of ribose, where one oxygen atom is replaced with a hydrogen atom. It is a component of DNA (deoxyribonucleic acid), the genetic material of organisms.

Monosaccharides can be further classified based on the number of carbon atoms they contain:

  • Triose: Monosaccharides with three carbon atoms. Example: glyceraldehyde.

  • Tetrose: Monosaccharides with four carbon atoms. Example: erythrose.

  • Pentose: Monosaccharides with five carbon atoms. Examples: ribose and deoxyribose (found in DNA and RNA).

  • Hexose: Monosaccharides with six carbon atoms. Examples: glucose, fructose, and galactose.

The carbohydrates  monomers(Monosaccharides) When two monosaccharides are chemically bonded together, they form disaccharides.

Monosaccharides  can be joined together by glycosidic bonds through a process called condensation or dehydration synthesis, where a molecule of water is removed, forming a glycosidic bond. This bonding results in the formation of larger carbohydrate molecules, including disaccharides and polysaccharides.

  1. Disaccharides: Disaccharides consist of two monosaccharide units joined together by a glycosidic bond. Some common examples include:

  • Sucrose: Sucrose, commonly known as table sugar, is composed of glucose and fructose units. It is found naturally in sugarcane, sugar beets, and various fruits. Sucrose is the most common form of sugar used in food and beverages.

  • Lactose: Lactose is the sugar found in milk and dairy products. It consists of a glucose unit joined to a galactose unit. Lactose is specific to mammalian milk and serves as an important source of energy for infants.

  • Maltose: Maltose is formed by the condensation of two glucose units. It is a product of the breakdown of starch and is found in germinating grains, such as barley and malted barley. Maltose is also used in brewing and baking processes.

  • There are other less common disaccharides as well, such as trehalose (formed by two glucose units) and cellobiose (formed by two glucose units in a different arrangement). These disaccharides have specific functions in various organisms and biological processes
  1. Polysaccharides: Polysaccharides are complex carbohydrates composed of long chains of monosaccharide units. They can be further classified into:

  • Starch: Starch is a polysaccharide found in plants and serves as a storage form of glucose. It consists of long chains of glucose units. Plants store starch in organs such as roots, tubers, and seeds. Humans and animals can digest starch through the action of enzymes like amylase, breaking it down into glucose for energy.
  • Glycogen: Glycogen is the storage form of glucose in animals, including humans. It is primarily stored in the liver and muscles. Glycogen is highly branched, allowing for rapid breakdown and release of glucose when energy demands increase, such as during exercise or fasting.
  • Cellulose: Cellulose is a structural polysaccharide found in the cell walls of plants. It consists of long chains of glucose units arranged in a way that makes it indigestible by most animals, including humans. Cellulose provides rigidity and strength to plant cell walls and is a major component of dietary fiber.
  • Chitin: Chitin is a polysaccharide found in the exoskeletons of arthropods (e.g., insects, crustaceans) and the cell walls of fungi. It is composed of modified glucose units with amino groups. Chitin provides structural support and protection to these organisms.
  • Peptidoglycan: Peptidoglycan is a unique polysaccharide found in the cell walls of bacteria. It consists of alternating sugars, N-acetylglucosamine (NAG), and N-acetylmuramic acid (NAM), cross-linked by short peptide chains. Peptidoglycan provides strength and rigidity to the bacterial cell wall.

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