Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
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Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
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Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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EXCRETION IN ANIMALS

Excretion is the process where metabolic wastes are eliminated from an organism. In humans, this function is performed through kidneys, lungs and skin. In animals, the main excretory products are:

  1. Ammonia
  2. Carbon Dioxide
  3. Urea
  4. Uric Acid
  5. Guanine
  6. Creatine

Modes of Excretion

Based on the excretory product, five modes of excretion are known in animals. They are:

  1. Ammonotelism (Type of excretion- ammonia)
  2. Ureotelism (Type of excretion – urea)
  3. Uricotelism (Type of excretion – uric acid)
  4. Aminotelism (Type of excretion – amino acids)
  5. Guanotelism (Type of excretion – guanine)

Ammonotelism

The process of eliminating ammonia from the body is known as ammonotelism , and the organisms which exhibit this nature are called ammonotelic. Most fish, protozoans, echinoderms, poriferans and crustaceans fall into this category. Aquatic animals excrete ammonia directly into the environment; where the compound is quickly diluted. It is also very toxic to tissues.

Ureotelism

In some mammals and amphibians, urea is excreted as the metabolic waste products. Such organisms are called ureotelic. In these organisms, ammonia that is produced is converted to urea in the liver of animals and is released back into the blood. The kidneys filter the urea and are expelled outside the body. Some of the urea is retained in the matrix of the kidney to maintain a desired osmolarity in the organisms. Humans are ureotelic as we expel the urea through urine. Moreover, urea is comparatively less toxic than ammonia.

Uricotelism

Uricotelic animals remove nitrogenous wastes as uric acid in the form of pellets or paste. Metabolically, this process is quite costly; however, the water loss is minimal, and it is the least toxic. Moreover, since uric acid is not readily soluble in water, the excrements form pasty white suspensions. Most reptiles, birds, and insects are classified as uricotelics.

Aminotelism

Certain molluscs and echinoderms excrete excess amino acids. This feature is called aminotelism.

Guanotelism

Spiders convert the ammonia into guanine before excretion. This characteristic is also found in some reptiles, birds and earthworms. It is also insoluble in water; hence no water is required for its excretion.

Excretion Examples & Structures

  1. All vertebrates have kidneys – Excretory product is urea
  2. Flame cells in planaria
  3. Earthworms have Nephridia
  4. Cockroaches have malpighian tubules
  5. Prawns have antennal glands or green glands
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