Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
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Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
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Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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Bones 

Bones provide the framework and internal core structure for the attachment of muscles. Bone is a living rigid tissue which forms the support structures for the rest of the body. The process of bone formation is called ossification. The matrix of bone contains a dense arrangement of collagen fibres together with mineral salts of calcium, magnesium and phosphates. The calcium salts give bone its hardness and rigidity while collagen fibres give bones its flexibility and strength.

Functions of bone

  1. To serve as a firm support framework for the whole body.
  2. To protect such delicate structures as the brain and spinal cord.
  3. To serve as levers, working with attached muscles to produce movement.
  4. To serve as a storehouse for calcium salts, which may be reabsorbed into the blood if there is not enough calcium in the diet.
  5. To produce blood cells in the red marrow.

Microscopic structure of a long bone

Bones are made up of numerous hollow tunnels called Haversian canals. Haversian canals occur within the matrix of bone tissue and run parallel to the length of the bone. Each Haversian canal consists of a nerve to carry impulses, blood vessels to transport gases, food and wastes and a lymph duct to drain tissue fluid. Under the microscope they appear as black circles against a lighter background.

Each Haversian canal is surrounded by concentric rings of compact bone called lamellae. Each of these layers contains a ring of fluid-filled cavities called lacunae. Each of these lacuna will contain a number of bone cells called osteocytes. The lacunae are linked to each other and to the Haversian canal by a system of very tiny interconnecting canals called canaliculi. Strands of cytoplasm extend through these canals which supply the osteocytes with oxygen and nutrients and remove waste products. The Haversian canals, lacunae, osteocytes and canaliculi together form a unit called an Haversian System and a number of these systems make up compact bones.

Apart from osteocytes which are embedded in the lacunae of bone there are two other types of bone cells.

Osteoblasts: Bone forming cells. These cells allow the bone to change and remodel its shape as the organism grows and responds to stresses. If a bone is broken or if strengthening is needed, bone cells lay down new tissue and repair damaged tissue

Osteoclasts: Special bone cells for destroying and reabsorbing bone tissue.

Structure of long bone

Although there are many different types of bones in the skeleton, we will discuss the different parts of a specific type of bone: the long bone. The femur, tibia and fibula in the leg, and the humerus, radius and ulna in the arm are all examples of long bones.

Epiphysis: The head of each end of a long bone consists largely of spongy bone and is covered with hyaline cartilage.

Spongy bone: Found in the epiphysis of long bones and contains red marrow.

Red bone marrow: Found in the spaces between the trabeculae in spongy bone. This is where the red blood cells are made at the rate of 2 -3 million per second. White blood cell types are also produced here.

Trabeculae: The struts in the network of irregular bony plates in the epiphysis of bones which transfer stresses from the epiphysis to the diaphysis which has a much thicker layer of compact bone and resists stress better.

Diaphysis: Cylindrical shaft of a long bone composed of hard compact bone on the outside.

Periosteum: The membrane of dense fibrous connective tissue which surrounds the outside surface of the shaft of a long bone. It has blood vessels which enables it to nourish the bone and repair injuries. It also provides a surface for the attachment of muscles by means of tendons and ligaments.

Endosteum: The delicate connective tissue layer lining the inside surface of compact bone.

Marrow cavity: This is filled with yellow marrow which consists largely of fat.

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