Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
0/8
Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
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Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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Nitrogen Cycle

The nitrogen cycle is the biogeochemical process by which nitrogen is converted and circulated through various forms in the environment. Nitrogen is an essential element for living organisms, and the nitrogen cycle ensures its availability and transformation. Here are the key steps of the nitrogen cycle:

  1. Nitrogen Fixation:

Nitrogen fixation is the process by which atmospheric nitrogen (N2) is converted into a usable form for plants and other organisms. This can occur through biological nitrogen fixation, where certain bacteria, such as Rhizobium, Azotobacter, and cyanobacteria, convert N2 into ammonia (NH3) or ammonium (NH4+). Some nitrogen-fixing bacteria form symbiotic relationships with plants, residing in root nodules and providing them with fixed nitrogen.

2. Nitrification:

Nitrification is the conversion of ammonia (NH3) or ammonium (NH4+) into nitrite (NO2-) and then into nitrate (NO3-). This process is carried out by nitrifying bacteria, primarily Nitrosomonas and Nitrobacter. Ammonia is oxidized to nitrite by Nitrosomonas, and nitrite is further oxidized to nitrate by Nitrobacter. Nitrate is the primary form of nitrogen that plants can readily absorb and utilize.

3. Assimilation:

Assimilation is the process by which plants and other organisms incorporate nitrogen compounds, particularly nitrate, into their own organic molecules, such as proteins, nucleic acids, and chlorophyll. Plants take up nitrate ions from the soil through their roots and convert them into amino acids and other nitrogen-containing compounds.

4. Ammonification:

Ammonification, also known as mineralization, is the process by which organic nitrogen compounds, such as dead plants and animal waste, are decomposed by bacteria and fungi. These decomposers break down complex organic nitrogen compounds into simpler inorganic forms, primarily ammonium (NH4+), releasing ammonia in the process.

5. Denitrification:

Denitrification is the conversion of nitrate (NO3-) back into nitrogen gas (N2), completing the nitrogen cycle. Denitrifying bacteria, such as Pseudomonas and Bacillus species, carry out this process in anaerobic conditions (lack of oxygen). These bacteria use nitrate as an alternative electron acceptor, converting it to nitrogen gas and releasing it back into the atmosphere.

6. Human Activities and Nitrogen Fixation:

Human activities, such as the use of synthetic fertilizers and the burning of fossil fuels, have significantly impacted the nitrogen cycle. The production and application of nitrogen-based fertilizers have increased the availability of fixed nitrogen in the environment. This excess nitrogen can lead to water pollution, as nitrate can leach into groundwater or run off into water bodies, causing eutrophication.

The nitrogen cycle is essential for maintaining the balance of nitrogen in ecosystems and supporting the growth and development of organisms. Human activities can disrupt this cycle and have ecological consequences. Understanding and managing the nitrogen cycle are important for sustainable agricultural practices, minimizing environmental pollution, and preserving ecosystem health.

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