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Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
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Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
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Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
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Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
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Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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Human skeleton 

Humans have a living endoskeleton (internal skeleton) made of bone, cartilage and connective tissue. At birth, the human skeleton consists of over 270 bones. However, in adults this number has reduced to 206 bones due the fusion of smaller bones into larger structures.).

Functions of skeleton 

The human skeleton is living and performs many functions in the body. Some important functions are summarised below:

  1. Movement:muscles attach to the bones of the skeleton, enabling movement.
  2. Protection:the skull protects the brain, the ribcage protects the heart and lungs, and the pelvic bones protect the digestive tract and reproductive organs.
  3. Support:provides shape and support to the body.
  4. Storage of minerals:bones store minerals such as calcium and phosphate ions.
  5. Hearing:bones in the middle ear, called the hammer, anvil and stirrup, amplify sound waves and assist in the hearing process.
  6. Red blood cell production:long bones and flat bones contain red bone marrow to produce red blood cells.

There are 206 bones in the adult human skeleton, which can be ‘grouped’ into different categories.

The adult skeleton is made up of the axial skeleton and the appendicular skeleton 

A Diagram showing an overview of the main skeletal features of the human skeleton.

Axial skeleton 

the axial skeleton forms the central axis of the body and consist of the skull, vertebral column, rib cage and sternum

The Skull

The skull consists of the cranium and facial bones.

The cranium consists of eight flat bones joined together by immovable joints called sutures. The cranium surrounds and protects the brain. There is a large opening at the base of the skull called the foramen magnum through which the spinal cord passes. On either side of the foramen magnum is a projection which articulates with the first vertebra (called the atlas) to allow for the nodding movement of the head.

                The cranium.

There are 15 facial bones. These are irregular bones that include the cheek bones, nasal bones, temples, upper jaw bone (maxilla)) and lower jaw bones (mandible). The only movable bone is the lower jaw. The upper and lower jaws bear the sockets for the 3232 permanent teeth.

The number, type and arrangement of the teeth in an animal is indicated by a dental formula. The human dental formula is: 2.1.2.3/2.1.2.3. This formula represents the numbers of each type of teeth in half of the upper jaw and half of the lower jaw. This formula tells us that in both the upper and lower halves there are 2 incisors, 1 canine, 2 premolars and 3 molars.

              The Dental formula in a human adult.

The human vertebral column

The vertebral column typically consists of 24 articulating vertebrae and 9 fused vertebrae in the sacrum and the coccyx. Between the vertebrae are discs of fibrocartilage, which prevent friction between vertebrae, and act as shock absorbers during walking, running and jumping. Spinal nerves are able to enter and leave the spinal cord through gaps between adjacent vertebrae. Strong ligaments and muscles around the spine stabilise the vertebrae and help to control movement. The vertebrae join up to each other in such a way that there is a continuous spinal canal which runs from the base of the skull to the pelvic girdle. This canal contains the spinal cord. The entire vertebral canal can be divided into five regions.

  1. Cervical region
  2. Thoracic region
  3. Lumbar region
  4. Sacral region
  5. Coccyx

                      The Human vertebral column.

Here are a few interesting facts about the human skeleton:

  1. Humans have seven neck bones – the same as giraffes.
  2. The strongest and longest bone is the thigh bone (the femur).
  3. One out of 20 people have an extra rib.

The cervical (neck) region consists of seven vertebrae. The first cervical vertebra, called the atlas, supports the skull and allows for the nodding movement of the head. The second vertebra, called the axis, has a projection on which the atlas pivots to give the side to side movement of the head.

The thoracic region (chest) consists of 12 vertebrae, which each bear a pair of ribs.

The lumbar region (lower back) consists of five vertebrae. This region has the largest vertebrae as it carries the weight of the body.

The sacral region consists of five fused vertebrae, forming a bone called the sacrum. the sacrum forms part of the pelvic girdle which provides surfaces for the attachment of muscles and the legs.

The coccyx is made up of four fused bones. These bones form the tail in those mammals that have tails.                                                                

 In a newborn baby the entire vertebral column curves backwards probably because of the confines of the uterus. Initially a baby cannot support the weight of its head. When after about 3 months it is able to support its head, the cervical forward curve is complete. The lumbar forward curve is complete when the baby is able to stand on its own and ready to learn to walk.

Functions of the vertebral column

  1. Supports the skull
  2. Surrounds and protects the spinal cord
  3. Provides attachment for ribs, girdles, and back muscles
  4. Separate vertebrae and S-shaped curvature provide flexibility allowing humans to bend backwards, forwards and sideways
  5. Fibrocartilage discs between the vertebrae act as shock absorbers

The rib cage and sternum

The rib cage is a bony and cartilaginous structure. A typical rib cage consists of 24 ribs (12 pairs), the sternum (an inverted T-shaped structure connecting the rib bones), costal cartilages and the 12 thoracic vertebrae shown in the diagram below. The first seven pairs of ribs connect directly to the sternum and are referred to as true ribs. The remaining five pairs of ribs do not connect directly to the sternum and are referred to as false ribs. The rib cage aids in the protection of the heart and lungs. With the help of the diaphragm and the intercostal muscles, they increase and decrease the volume of the thoracic cavity thereby allowing inhalation and exhalation to take place.

                           The human rib cage.

Appendicular Skeleton

The appendicular skeleton consists of the pectoral girdle with the arms and the pelvic girdle with the legs. The pectoral girdle and arms and pelvic girdle and legs will be explored in greater detail in the following section.

The appendicular skeleton is shaded in red.

The pectoral girdle and arms

The pectoral girdle consists of two clavicles (collar bones) and two scapulae (shoulder blades). Each clavicle is attached to the sternum in the front and the scapulae at the sides and they help to support the shoulders. The clavicle is the most frequently broken bone in the body as it often takes the full impact of falls on outstretched arms or of blows to the shoulder. The pectoral girdle is connected by muscles to the back of the thorax to enable it to have a supporting structure as well as giving the shoulders greater freedom of movement which in turn allows greater mobility of the arms. Any limit to movement is provided by the clavicle.

Each upper arm has a single bone called the humerus which fits into the Glenoid cavity on the scapula to form a ball and socket joint. This cavity is very shallow which allows the arms to move in almost any direction. The forearm consists of two bones namely the ulna in line with the little finger and the radius in line with the thumb. The joint at the elbow is a hinge joint. The wrist consists of eight small carpal bones arranged in two rows of four. The palm of the hand consists of five metacarpal bones. There are 14 digits (short bones) or phalanges in each hand, two in each thumb and three in each of the fingers.

Skeletal framework of the arm and shoulder region.

Functions of pectoral girdle

  1. Forms a strong support structure for the attachments of the arms.
  2. Provides large area of bone for the attachment of muscles.
  3. Forms ball-and-socket joints with the arms which allows the arms to move freely.

Pelvic girdle and the legs

The pelvic girdle consists of hip bones joined at the front by cartilage called the pubic symphysis and they are attached to the sacrum at the back. Each hip bone consists of three fused bones (ilium, ischium and pubis). Portions of all three bones contribute to the formation of the acetabulum, a deep socket into which the head of the femur (thigh bone) joins to form the hip joint.

Skeletal framework of pelvic girdle.

The female pelvic girdle is wider and lighter than the male. This is an adaptation to allow for pregnancy and childbirth.

The femur in the leg is the largest and strongest bone in the body. The upper end forms a ball and socket joint with the hip bone while the lower end articulates with the tibia to form the hinge joint of the knee. The patella or kneecap is a flat triangular bone which is embedded in the tendon of the thigh muscle and attached by a ligament to the tibia.

There are two bones in the lower leg: the tibia (shin bone) which is the larger of the two and supports most of the mass. The upper end articulates with the femur while the lower end articulates with one of the tarsal bones to form the ankle joint. The fibula (calf bone) is thinner than the tibia and serves mainly for the attachment of muscles. It is attached to the femur and is articulated to the top and bottom of the tibia.

Skeletal framework of pelvic girdle and legs.

The structure of the foot is similar to that of the hand. However, the foot supports the weight of the body, so it is stronger and less mobile than the hand. There are seven tarsals or ankle bones, only one of which, the talus, articulates with the tibia. The talus us also know as the ankle bone. The heel bone (calcaneum) is the largest of the tarsal bones and is the bone to which the calf muscle is attached. The heel bone presses firmly on the ground when one stands, walks or runs.

there are 5 metatarsal bones which form the ball and arch   of the foot. The 14 phalanges of the toes are  are the counterparts of those of the fingers, with the big toe having two phalanges and the other four having 3 phalanges each. Together, these structures consist of the bones form the lower limb as shown below

 The Bones of lower extremity.


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