Course Content
Laboratory Design and Layout
In this topic. we will; 1.Discuss the importance of laboratory design and layout. 2.Describe standards for a good science laboratory. 3.Describe the essential features of a prep room and a laboratory store. 4.Describe the basic laboratory services
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Laboratory Safety and Housekeeping
Objectives To highlight the importance of laboratory housekeeping practices and how its proper integration into laboratory management can: 1.Create order and good organization 2.Increase safety 3.Increase efficiency 4.In this chapter, we will discuss 5.The importance of laboratory housekeeping 6.Ways of enhancing good laboratory housekeeping practices
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Personal Protective Equipments
OBJECTIVES The objective of this topic is to highlight the importance of protective equipment’s and how its proper integration into laboratory management can to reduce: 1.Injury and deaths to laboratory users 2.Contamination of laboratory reagents and specimens  In this chapter, we will 1.Discuss the importance of laboratory protective equipment. 2.Describe the various types of laboratory protective equipment 3.Describe how to use laboratory protective equipment’s. 4.Describe how to care for laboratory protective equipment’s
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Laboratory Hazards
OBJECTIVES The objective of this chapter is to highlight the importance of laboratory hazards, how to identify and get rid (or minimize) of them from the laboratory. In this chapter, we will 1.Describe the various types of laboratory hazards and. 2.Describe causes of laboratory hazards 3.Describe the general ways to avoid laboratory hazards.
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First Aid
OBJECTIVES The objective of this chapter is to prepare you as a first aider, psychologically and emotionally, as well as by giving practical advice on what you should and should not do in a laboratory emergency. After studying the material in this chapter, you will be able to: 1.Define first aid 2.Recognize the reasons why first aid is given 3.Manage an emergency situation safely 4.Assess the sick or injured persons. 5.Identify general directions for giving first aid
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Laboratory Apparatus
This topic focuses on the importance of handling, care and cleaning of laboratory wares. In this chapter, we will 1.Classify the various laboratory wares. 2.Discuss use of various laboratory wares 3.Describe various cleaning methods for various laboratory stains 4.Describe the various laboratory cleaning agents
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Laboratory Cleaning
OBJECTIVES The objective of this topic is to learn the importance of ensuring general and overall cleanness of laboratory items and surfaces. In this chapter, we will: 1.Discuss the various types of dirt and stains. 2.Discuss the general methods of cleaning laboratory apparatus and surfaces. 3.Describe various types cleaning agents used for cleaning laboratory items. 4.Outline preparation of laboratory cleaning agents. 5.Discuss various methods used in sterilization and disinfections of laboratory items.
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Sterilization Techniques
Sterilization, also called biological cleaning is a bit more involving than chemical cleaning. This is because it involves total killing of microorganism’s present on the apparatus. There are two methods used in sterilization. 1.Physical methods 2.Chemical methods
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Laboratory Waste Management
OBJECTIVES This chapter presents methods for the management and ultimate disposal of laboratory waste that may present as hazards. The objectives of these chapter are b.To identify types of laboratory wastes c.To identify sources of laboratory wastes d.To describe methods of managing laboratory wastes
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Laboratory Chemicals and Reagents
OBJECTIVES The objectives of this chapter are to learn the importance of proper handling, preparation, storage and disposal of laboratory chemicals and reagents. In this chapter, we will 1.Discuss the various categories of laboratory reagents. 2.Discuss the general storage guidelines for laboratory chemicals. 3.Describe in details the various methods of handling, storage, and disposal of laboratory chemicals. 4.Discuss how to segregate non compatible laboratory chemicals. 5.Discuss the basic terms and concepts of preparing Solutions. 6.Describe how to prepare various laboratory reagents and solutions
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Laboratory Solution Preparation
OBJECTIVES The objectives of these topic are: 1.To define terms used in solution preparation 2.To prepare various concentrations of solutions 3.To prepare bench reagents
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Laboratory Equipments
OBJECTIVES The objective of this chapter are to give a better understanding of the technical requirements regarding installation, use and maintenance of various types of equipment which play an important role in performing laboratory tests. In this chapter, we will 1.Identify the various types of laboratory equipment’s 2.Discuss the use of the equipments. 3. Describe the components of the equipments. 4.Discuss the operational mechanisms of the equipments 5.Outline the general care and maintenance for the equipments
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Cryogenic Technology
Cryogens are substances (liquids or fluids) usually at very extreme low temperatures (below –150 °C, –238 °F or 123 K). Cryogenic technology can be defined as the study of the production of low temperature fluids, measurements at low temperatures, and behavior of these materials at low temperature and how to produce them. It is also concerned with the application of low temperature processes and techniques
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Glass Blowing Technology
OBJECTIVES In this chapter, we will 1.Define the term glassblowing 2.Discuss importance of glassblowing in the laboratory 3.Describe composition and properties of glass used for glassblowing 4.Describe various tools used for glassblowing 5.Describe glass blowing techniques used in making simple glass apparatus 6.Discuss various conditions encountered in glass blowing 7.Discuss how to correct conditions encountered in glass blowing
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Vacuum Technology
OBJECTIVES In this chapter, we will 1.Define the term vacuum and degrees of vacuum 2.Discuss types of vacuum system 3.Describe how vacuum is created 4.Describe various types of vacuum pumps 5.Describe instruments for measuring vacuums 6.Discuss leaks and leaks detection in vacuum systems 7.Discuss materials used in vacuum system construction 8.Discuss vacuum cleaning techniques 9.Discuss applications of vacuums
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Photographic Technology
Photography simply means writing, drawing or printing with light. Light is therefore most fundamental in photography because without it then no photographs will be produced. Light is a form of energy, it’s a source of all colors and its composed of different wavelengths, those that are of importance in photography are those that fall within the visible region of the electromagnetic spectra because they can be perceived by the human eye. These are composed of different colors e.g. red, blue green yellow orange etc.
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Laboratory Inventory Management
OBJECTIVES The objective of this chapter is to give a better understanding of good inventory management practices . In this chapter, we will: 1.Describe the importance of good laboratory stock management practices 2.Define the various terms used in inventory management 3.Discuss ways to monitor, control and record laboratory stock
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Laboratory Inspection
The laboratory inspection is the safety audit of the conditions and operations that occur on a daily basis in a specific laboratory. Laboratory inspections are conducted at least annually to ensure safety and compliance with all applicable lab safety regulations and guidelines. These inspections are also intended to provide a formal opportunity for laboratory personnel to ask questions regarding any aspect of lab safety in order to improve overall compliance. Laboratory inspectors need to have a greater understanding of safe laboratory operations. The lab inspector needs a higher level of laboratory safety training to be able to recognize problem areas and be able to ask questions and eliminate the hazards.
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Laboratory Management
The objectives of this topic are to; 1.Describe what management is. 2.Explain the primary functions of management. 3.Describe the primary types of managers and the roles they play.
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Principles of Laboratory Management
OBJECTIVES The objectives of this topic are to; 1.Describe what management is. 2.Explain the primary functions of management. 3.Describe the primary types of managers and the roles they play.
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Schools and Theories of Management
The objectives of this topic are 1. To understand the way in which management thought has evolved in the West since 1900; 2. To appreciate how these 'theories' may be applied today; 3. To compare and contrast the various theories presented
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Management Functions
OBJECTIVES 1. To describe the process of management's five functions: planning, organizing, staffing. leading, and controlling.
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Delegation
Delegation is the process by which managers assign a portion of their total workload to others. It includes assigning formal authority and responsibility for completion of specific activities.
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Line and Staff Relationship
The line and staff relationship is a form of organizational structure that exists in many companies. It refers to the relationship between line departments (also known as line units) and staff departments (also known as staff units).
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Centralization and Decentralization
This refers to the extent to which decision-making power and authority is dispersed to lower levels. It also refers to the degree of delegation of duties, power and authority to lower levels of an organization.
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Span of Control
Span of control management refers to the number of subordinates who report directly to a given superior.
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Leadership
Leadership is an important aspect of management and the ability to lead is one of the keys to being an effective manager. Leadership is the art or process of influencing people so that they will strive willingly and enthusiastically toward achievement of group goals. Effective leaders must be able to influence the thoughts and behavior of others towards the accomplishment of some goals or goal.
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Motivation
One of the most important functions of management is to create willingness amongst the employees to perform in the best of their abilities. Therefore the role of a leader is to arouse interest in performance of employees in their jobs through motivating them to act in the desired direction.
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Effective Communication
Effective communication is a process of exchanging ideas, thoughts, knowledge and information such that the purpose or intention is fulfilled in the best possible manner.
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Management Styles
OBJECTIVES The objectives of these topic are 1.Define the various types of management styles. 2.To discuss pros and cons of management styles. 3. Explain how we can determine which style to use in a variety of situations.
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Labor Laws and Industrial Relationships
Labor laws refers to rules and regulations are put in place to govern the working relationship and protect the rights of employers and workers by clearly stipulating the obligations and responsibilities of each party to the employment. They are enforced by the Kenyan government agencies to ensure that there is harmony in the workplace
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Woodwork Workshop Practice
Woodwork, also known as woodworking, is the craft or skill of creating objects or structures from wood using various tools and techniques. Woodwork encompasses a wide range of activities, from simple woodworking projects to complex furniture making and carpentry. It combines both practical skills and creativity to transform raw wood into functional or decorative items.
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Metalwork Workshop Practice
Metalwork refers to the craft or skill of working with metal to create objects, structures, or components. It involves various processes, techniques, and tools to shape, manipulate, and join metal materials.
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Plastics
Plastics are a group of synthetic materials made from polymers, which are large molecules composed of repeating subunits called monomers. Plastics have a wide range of applications due to their versatility, durability, lightweight nature, and ability to be molded into various shapes.
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Laboratory Practice and Management
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GLASS BLOWING PROCEDURE

Cutting of glass tubing

  • Make a short etched line at the point where the glass tubing is to be cut   using a triangular file.

(i) To break the tubing at the etched mark, the tubing is held in both hands with the thumb placed on each side of the mark and close to the mark.

(ii) The glass is then pulled slightly towards its ends at the same time broken away from the body in one Motion.

NB: This procedure is only suitable for glassware which are less than 10mm in diameter. For tubing with over10mm diameter, the following procedures should be adopted.

  1. Etch the entire circumference at the desired line of cleavage, taking care that the two ends of the line meet in a complete circle.
  2. This scored line can also be tied with a piece of thread soaked in a suitable flammable solvent and then lighted.
  3. The glass cracks around the line and the two ends can be pulled apart.
  4. After glass tubing has been cut, the sharp edges should be flame polished in order to make them smooth.

Bending of glass tubing  

(i) Formation of L – bends

L-bend glass tubing is used to convey   liquids or gases from one container or flask to another .bends should be well rounded so that the tubing retains their original diameters. , A burner with a wide flame is generally used for the bending of the glass tubing. A tube is heated while holding it with both hands in length so that 5-8 cm area is heated while making sure that it is evenly and slowly rotated about its axis so as to heat all sides equally.

As soon as the glass is felt to be soft enough it’s slowly bent to the required shape. These  is done by  removing it from the flame  and allowing one end to fall gradually under its own weight  while being guided  so that it is in the same plane  as the rest of the tube.

The glass tubing must not be forced to bend otherwise a bend with a kink will be formed

Joining two straigt tubes or Butt Seals

    Butt Sealing is the process of joining two sections of glass tubing together – end to end. This exercise demonstrates the joining of two similar size tubes together.  A variation of this seal  would be the joining of two dissimilar size tubes. Two options are offered – one requires good rotational skills.
1. Cut tubing into 8″ – 12″ lengths, fire polish ends.

  1. Attach blowhose assembly to one tube.
  2. Seal second tube with a cork or use a test tube bottom from the previous lesson.
  3. Light torch and adjust flame.
  4. Rotate ends of glass tubing (as if fire polishing) in flame.
    6. When tube ends are red hot remove from flame, stop rotating, push tubes together making contact all around.

Another Option 

This option does not require rotation. It does require the glassblower to hold and manipulate the torch around the glass tube that is held in a standard laboratory ring stand.

1. Clamp tube #1 vertically in a ring stand. Tube end about chin high with blow hose attached.

  1. Hold in one hand the torch adjusted to a sharp flame. A sealed tube (#2) is held in the other hand.
  2. Sweep flame back and forth across the end of tube #1, concentrating on the back half of the tube.
  3. Tube #2 is simultaneously held directly above tube #1 at a slight angle. This tube will also be heated as in step 3.
  4. Bring tube #2 down contacting the back of tube #1, continue to sweep flame back and forth across the tube ends.
  5. Continue to bring tube #2 down onto #1 making complete contact around the circumference. With practice steps 5 and 6 are completed in a quick smooth continuous motion.
  6. Using a small sharp flame, work out the seal by heating small sections of the seal at a time.

8.Anneal

(iii) Joining two bulbs of different diameters

The larger diameter tubing is sealed at one end with rubber tubing and the other end is rotated in the flame until the bore is the same diameter as that of the

smaller tube. One end of the smaller tube is then gently heated and the two hot ends are joined together. The flame is reduced and the junction is rotated over it.

                                        Heat here

When red hot, the tubing is removed and the free end is gently blown to form a small bulb at the junction. The process is repeated until the two tubes are fused together, but when fusion is complete, the two tubes are pulled slightly apart to cause slight tempering at the junction.

( c) Blowing of bulbs

(i) End bulbs

Place the required end of the bulb on the flame and rotate until it becomes sealed .remove from the flame and blow while still hot through the other end until a small bulb appears.

                                              End bulb

Using a large flame, reheat the bulb and a portion of the adjacent tube and gently blow again when it’s soft. End bulbs can be used to make thermometers.

(ii) Center bulbs

Stopper one end and heat the middle of the tubing until it’s soft and red. Remove it from the flame and press both ends towards the center to form a ring.

Heat the ring formed until its red-hot and blow. Repeat the process until the bulb formed reaches the required size.

                         Center bulb

T and Y joints (T seals)

  T seals are simply two tubes sealed together at right angles. Modified T seals may have the glass tubes at any angle degree, the basic technique being the same. Joining dissimilar sized tubes end to end may be more appropriately classified as butt seals, but for our purposes are carried in this section because the technique shown is similar to the T seal.
1. Clamp a sealed 10 mm tube horizontally in a ring stand. Attach blowhose assembly to tube.

  1. Light and adjust torch to a sharp flame. Remove from holder.
  2. Heat a 10 mm spot on top of tube.
  3. Remove torch flame – blow out a bubble about 10 mm in diameter (do not pop).
  4. Reheat bubble and let glass collapse to a flat even plateau, remove torch. Sides of plateau should be about 3 mm high.
    6. Reheat plateau, remove torch and blow a second bubble, (do not pop).
    7. Position flame to cross over the top of the bubble, blowing hard at the same time to pop bubble.
  5. Melt glass evenly down, forming a hole 10 mm in diameter with level 3 mm high side walls.
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