Course Content
Matter
OBJECTIVES By the end of this topic, the trainee should be able to 1.Define matter 2.Explain state of matter 3.Distinguish between physical and chemical changes 4.Explain the gas laws
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Atoms , Elements and Compounds
OBJECTIVES By the end of this topic , the trainee should be able to; 1.Define Elements, Compounds and Mixtures 2.Describe the structure of an atom 3.Describe how to determine the Atomic number ,Mass number and Isotopes
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The Periodic Table
OBECTIVES By the end of this topic, the trainee should be able to : 1.State the historical contribution on development of the periodic table 2.Explain the periodic trends of elements and their compounds 3.State the diagonal relationships of the periodic table
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The S-Block Element
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain the chemistry of group I and II elements 2.State the application of group I and two elements and their compounds
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Chemical Bonds
OBJECTIVES By the end of these topic, the trainee should be able to 1.Identify different types of bonds 2.Describe their properties
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Chemical Equilibrium
OBJECTIVES By the end of this topic , the trainee should be able to : 1.Define chemical equilibria 2.Explain types of equilibria 3.Determine equilibrium constant 4.Describe factors affecting chemical equilibrium
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Introduction To Organic Chemistry
By the end of this topic , the trainee should be able to : 1.Explain the aspects of organic chemistry 2.Describe hydrocarbons 3.Classify organic molecules explain chemical reactions of simple organic molecules 4.Explain the properties , synthesis and uses of simple organic molecules
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Acids, Bases and Salts
OBJECTIVES By the end of this session , the trainee should be able to : 1.State properties of acids and bases 2.Differentiate between strong and weak acids 3.Explain types and properties of salts
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PH Analysis
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define the term PH 2.Explain the basic theory of PH 3.State the relationship between PH and color change in indicators 4.Explain the term buffer solution 5.Describe the preparation of buffer solutions 6.State the application of buffer solutions
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Sampling and Sample Preparation
OBJECTIVE By the end of this topic, the trainee should be able to : 1.Define the terms used in sample preparation 2.State the importance of sampling 3.Describe the techniques of sampling 4.Describe the procedure for sample pre-treatment 5.State sample storage methods
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Separation Techniques
OBJECTIVES By the end of this topic , the trainee should be able to : 1.Define separation, extraction and purification 2.Describe the separation , extraction and purification techniques 3.Explain the methods of determining purity of substances
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Heating and Cooling Techniques
OBJECTIVES To identify various techniques used for heating and cooling substances in the laboratory
Heating and Cooling Techniques
OBJECTIVES To identify various techniques used for heating and cooling substances in the laboratory
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Distillation Techniques
By end of this topic, Trainee should be able to : 1. Define distilation 2. State and explain various distillation techniques 3. Outline Various distillation techniques 4. Outline the applications of Distillation techniques
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Crystallization Techniques
OBJECTIVES By the end of the topic, the learner should be able to: 1.To define crystallization 2.To describe crystallization process 3.To carry out crystallization procedure
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Solvent Extraction Techniques
OBJECTIVES By the end of the topic, the learner should be able to 1.Define solvent extraction 2.Explain terms used in solvent extraction 3.Describe methods of solvent extraction 4.Describe selection of appropriate solvents for solvent extraction 5.Determine distribution ration 6.Outline factors actors influencing the extraction efficiency 7.Describe Soxhlet extraction
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Chromatography Techniques
OBJECTIVES By the end of this topic, the learner should be able to: 1.Define chromatography techniques 2.Explain terms used in chromatography techniques 3.Describe principles of chromatography techniques 4.Explain types of chromatography techniques 5.Carry out chromatography experiments 6.Determine RF factor 7.Outline electrophoresis
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Titrimetric Analysis
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms used in titrimetric analysis 2.Describe types of titrimetric analysis 3.Balance chemical reactions 4.Work out calculations involved in titrimetric analysis
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Redox Titration
Redox Titration is a laboratory method of determining the concentration of a given analyte by causing a redox reaction between the titrant and the analyte. Redox titration is based on an oxidation-reduction reaction between the titrant and the analyte. It is one of the most common laboratory methods used to identify the concentration of unknown analytes. Redox reactions involve both oxidation and reduction. The key features of reduction and oxidation are discussed below.
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Complexiometric Titration
omplexometric Titration or chelatometry is a type of volumetric analysis wherein the colored complex is used to determine the endpoint of the titration. The method is particularly useful for determination of the exact number of a mixture of different metal ions, especially calcium and magnesium ions present in water in solution .
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Gravimetric Analysis
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define gravimetric analysis 2.Describe the principles of gravimetric analysis 3.Describe the steps involved in gravimetric analysis 4.Explain factors affecting gravimetric analysis 5.Describe the equipments and apparatus used in gravimetric analysis 6.Carry out gravimetric analysis
0/8
Calorimetric Analysis
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms and units used in thermochemistry 2.Determine enthalpy changes in chemical reactions 3.Determine heat capacity and specific heat capacity 4.Compare calorific values of different materials 5.Determine different heat reactions 6.Apply law of conservation of energy and Hess law in thermochemical calculations
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Chemistry Techniques for Science Laboratory Technicians
About Lesson

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TYPES OF SAMPLES

Sampling techniques can broadly be classified into two categories namely;

  1. Probability sampling
  2. Non probability sampling

Non-probability Sampling:

Non probability sampling method is reliant on a researcher’s ability to select members at random. This sampling method is not a fixed or pre-defined selection process which makes it difficult for all elements of a population to have equal opportunities to be included in a sample.

Probability Sampling Methods

Probability sampling is a sampling method that selects random members of a population by setting a few selection criteria. These selection parameters allow every member to have the equal opportunities to be a part of various samples.

Probability Sampling is a sampling technique in which sample from a larger population are chosen using a method based on the theory of probability. This sampling method considers every member of the population and forms samples on the basis of a fixed process. For example, in a population of 1000 members, each of these members will have 1/1000 chances of being selected to be a part of a sample. It gets rid of bias in the population and gives a fair chance to all members to be included in the sample.

There are 4 types of probability sampling technique:

  1. Simple Random Sampling:

It is one of the best probability sampling techniques that helps in saving time and resources. It is a trustworthy method of obtaining information where every single member of a population is chosen randomly, merely by chance and each individual has the exact same probability of being chosen to be a part of a sample.

Cluster Sampling:

Cluster sampling is a method where the researchers divide the entire population into sections or clusters that represent a population. Clusters are identified and included in a sample on the basis of defining demographic parameters such as age, location, sex etc. which makes it extremely easy for a survey creator to derive effective inference from the feedback.

For example, if the government of the Kenya wishes to evaluate the number of students TVET institutions   they can divide it into clusters on the basis of national polytechnics, technical institutes, vocational institutions etc. This way of conducting a survey will be more effective as the results will be organized into institutions and provides insightful student data.

Systematic Sampling:

Using systematic sampling method, members of a sample are chosen at regular intervals of a population. It requires selection of a starting point for the sample and sample size that can be repeated at regular intervals. This type of sampling method has a predefined interval and hence this sampling technique is the least time-consuming.

For example, a researcher intends to collect a systematic sample of 500 people in a population of 5000. Each element of the population will be numbered from 1-5000 and every 10th individual will be chosen to be a part of the sample (Total population/ Sample Size = 5000/500 = 10).

Stratified Random Sampling:

Stratified Random sampling is a method where the population can be divided into smaller groups, that don’t overlap but represent the entire population together. While sampling, these groups can be organized and then draw a sample from each group separately.

For example, a researcher looking to analyze the characteristics of people belonging to different annual income divisions, will create strata (groups) according to annual family income such as  

Less than ksh 20,000,

Ksh 21,000 – 30,000,

Ksh 31,000 to 40,000,

Ksh 41,000 to 50,000 etc.

and people belonging to different income groups can be observed to draw conclusions of which income strata have which characteristics.

Use of the Probability Sampling Method

There are multiple uses of the probability sampling method. They are:

  1. Reduce Sample Bias: Using the probability sampling method, the bias in the sample derived from a population is negligible to non-existent. The selection of the sample largely depicts the understanding and the inference of the researcher. Probability sampling leads to higher quality data collection as the population is appropriately represented by the sample.
  2. Diverse Population: When the population is large and diverse, it is important to have adequate representation so that the data is not skewed towards one demographic. For example, if Square would like to understand the people that could their point-of-sale devices, a survey conducted from a sample of people across US from different industries and socio-economic backgrounds, helps.
  • Create an Accurate Sample: Probability sampling helps the researchers plan and create an accurate sample. This helps to obtain well-defined data.

Non-probability Sampling Methods

The non-probability method is a sampling method that involves a collection of feedback on the basis of a researcher sample selection capabilities and not on a fixed selection process. In most situations, output of a survey conducted with a non-probable sample leads to skewed results, which may not totally represent the desired target population. But there are situations such as the preliminary stages of research or where there are cost constraints for conducting research, where non-probability sampling will be much more effective than the other type.

There are 4 types of non-probability sampling which will explain the purpose of this sampling method in a better manner:

  1. Convenience sampling:This method is dependent on the ease of access to subjects such as surveying customers at a mall or passers-by on a busy street. It is usually termed as convenience sampling, as it’s carried out on the basis of how easy is it for a researcher to get in touch with the subjects. Researchers have nearly no authority over selecting elements of the sample and it’s purely done on the basis of proximity and not representativeness. This non-probability sampling method is used when there are time and cost limitations in collecting feedback. In situations where there are resource limitations such as the initial stages of research, convenience sampling is used.
  2. Judgmental or Purposive Sampling: In judgmental or purposive sampling, the sample is formed by the discretion of the judge purely considering the purpose of study along with the understanding of target audience. Also known as deliberate sampling, the participants are selected solely on the basis of research requirements and elements who do not suffice the purpose are kept out of the sample. For instance, when researchers want to understand the thought process of people who are interested in studying for their master’s degree. The selection criteria will be: “Are you interested in studying for Masters in …?” and those who respond with a “No” will be excluded from the sample.
  3. Snowball sampling:Snowball sampling is a sampling method that is used in studies which need to be carried out to understand subjects which are difficult to trace. In such cases, using the snowball theory, researchers can track a few of that particular category to interview and results will be derived on that basis. This sampling method is implemented in situations where the topic is highly sensitive and not openly discussed such as conducting surveys to gather information about HIV Aids. Not many victims will readily respond to the questions but researchers can contact people they might know or volunteers associated with the cause to get in touch with the victims and collect information.
  4. Quota sampling:In Quota sampling, selection of members in this sampling technique happens on basis of a pre-set standard. In this case, as a sample is formed on basis of specific attributes, the created sample will have the same attributes that are found in the total population. It is an extremely quick method of collecting samples.

Use of the Non-Probability Sampling Method

There are multiple uses of the non-probability sampling method. They are:

  1. Create a hypothesis: The non-probability sampling method is used to create a hypothesis when limited to no prior information is  available. This method helps with immediate return of data and helps to build a base for any further research.
  2. Exploratory research: This sampling technique is widely used when researchers aim at conducting qualitative research, pilot studies or exploratory research.
  • Budget and time constraints: The non-probability method when there are budget and time constraints and some preliminary data has to be collected. Since the survey design is not rigid, it is easier to pick respondents at random and have them take the survey or questionnaire.
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