Course Content
Matter
OBJECTIVES By the end of this topic, the trainee should be able to 1.Define matter 2.Explain state of matter 3.Distinguish between physical and chemical changes 4.Explain the gas laws
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Atoms , Elements and Compounds
OBJECTIVES By the end of this topic , the trainee should be able to; 1.Define Elements, Compounds and Mixtures 2.Describe the structure of an atom 3.Describe how to determine the Atomic number ,Mass number and Isotopes
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The Periodic Table
OBECTIVES By the end of this topic, the trainee should be able to : 1.State the historical contribution on development of the periodic table 2.Explain the periodic trends of elements and their compounds 3.State the diagonal relationships of the periodic table
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The S-Block Element
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain the chemistry of group I and II elements 2.State the application of group I and two elements and their compounds
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Chemical Bonds
OBJECTIVES By the end of these topic, the trainee should be able to 1.Identify different types of bonds 2.Describe their properties
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Chemical Equilibrium
OBJECTIVES By the end of this topic , the trainee should be able to : 1.Define chemical equilibria 2.Explain types of equilibria 3.Determine equilibrium constant 4.Describe factors affecting chemical equilibrium
0/6
Introduction To Organic Chemistry
By the end of this topic , the trainee should be able to : 1.Explain the aspects of organic chemistry 2.Describe hydrocarbons 3.Classify organic molecules explain chemical reactions of simple organic molecules 4.Explain the properties , synthesis and uses of simple organic molecules
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Acids, Bases and Salts
OBJECTIVES By the end of this session , the trainee should be able to : 1.State properties of acids and bases 2.Differentiate between strong and weak acids 3.Explain types and properties of salts
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PH Analysis
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define the term PH 2.Explain the basic theory of PH 3.State the relationship between PH and color change in indicators 4.Explain the term buffer solution 5.Describe the preparation of buffer solutions 6.State the application of buffer solutions
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Sampling and Sample Preparation
OBJECTIVE By the end of this topic, the trainee should be able to : 1.Define the terms used in sample preparation 2.State the importance of sampling 3.Describe the techniques of sampling 4.Describe the procedure for sample pre-treatment 5.State sample storage methods
0/5
Separation Techniques
OBJECTIVES By the end of this topic , the trainee should be able to : 1.Define separation, extraction and purification 2.Describe the separation , extraction and purification techniques 3.Explain the methods of determining purity of substances
0/2
Heating and Cooling Techniques
OBJECTIVES To identify various techniques used for heating and cooling substances in the laboratory
Heating and Cooling Techniques
OBJECTIVES To identify various techniques used for heating and cooling substances in the laboratory
0/1
Distillation Techniques
By end of this topic, Trainee should be able to : 1. Define distilation 2. State and explain various distillation techniques 3. Outline Various distillation techniques 4. Outline the applications of Distillation techniques
0/3
Crystallization Techniques
OBJECTIVES By the end of the topic, the learner should be able to: 1.To define crystallization 2.To describe crystallization process 3.To carry out crystallization procedure
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Solvent Extraction Techniques
OBJECTIVES By the end of the topic, the learner should be able to 1.Define solvent extraction 2.Explain terms used in solvent extraction 3.Describe methods of solvent extraction 4.Describe selection of appropriate solvents for solvent extraction 5.Determine distribution ration 6.Outline factors actors influencing the extraction efficiency 7.Describe Soxhlet extraction
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Chromatography Techniques
OBJECTIVES By the end of this topic, the learner should be able to: 1.Define chromatography techniques 2.Explain terms used in chromatography techniques 3.Describe principles of chromatography techniques 4.Explain types of chromatography techniques 5.Carry out chromatography experiments 6.Determine RF factor 7.Outline electrophoresis
0/6
Titrimetric Analysis
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms used in titrimetric analysis 2.Describe types of titrimetric analysis 3.Balance chemical reactions 4.Work out calculations involved in titrimetric analysis
0/6
Redox Titration
Redox Titration is a laboratory method of determining the concentration of a given analyte by causing a redox reaction between the titrant and the analyte. Redox titration is based on an oxidation-reduction reaction between the titrant and the analyte. It is one of the most common laboratory methods used to identify the concentration of unknown analytes. Redox reactions involve both oxidation and reduction. The key features of reduction and oxidation are discussed below.
0/5
Complexiometric Titration
omplexometric Titration or chelatometry is a type of volumetric analysis wherein the colored complex is used to determine the endpoint of the titration. The method is particularly useful for determination of the exact number of a mixture of different metal ions, especially calcium and magnesium ions present in water in solution .
0/5
Gravimetric Analysis
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define gravimetric analysis 2.Describe the principles of gravimetric analysis 3.Describe the steps involved in gravimetric analysis 4.Explain factors affecting gravimetric analysis 5.Describe the equipments and apparatus used in gravimetric analysis 6.Carry out gravimetric analysis
0/8
Calorimetric Analysis
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms and units used in thermochemistry 2.Determine enthalpy changes in chemical reactions 3.Determine heat capacity and specific heat capacity 4.Compare calorific values of different materials 5.Determine different heat reactions 6.Apply law of conservation of energy and Hess law in thermochemical calculations
0/4
Chemistry Techniques for Science Laboratory Technicians
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Buffer Solutions

Buffer solutions  are  solutions that resist change in hydronium ion and the hydroxide ion concentration (and consequently pH) upon addition of small amounts of acid or base, or upon dilution. Buffer solutions  consist of a weak acid and its conjugate base (more common) or a weak base and its conjugate acid (less common). The resistive action is the result of the equilibrium between the weak acid (HA) and its conjugate base (A):

          HA(aq) + H2O(l) ↔ H3O+(aq) + A(aq)

Any alkali added to the solution is consumed by the hydronium ions. These ions are mostly regenerated as the equilibrium moves to the right and some of the acid dissociates into hydronium ions and the conjugate base. If a strong acid is added, the conjugate base is protonated, and the pH is almost entirely restored. This is an example of Le Chatelier’s principle and the common ion effect. This contrasts with solutions of strong acids or strong bases, where any additional strong acid or base can greatly change the pH. This may be easier to see by comparing two graphs when an strong acid is titrated with a strong base the curve will have a large gradient throughout showing that a small addition of base/acid will have a large effect compared to a weak acid/strong base titration curve which will have a smaller gradient near the pKa. When writing about buffer systems they can be represented as salt of conjugate base/acid, or base/salt of conjugate acid. It should be noted that here buffer  are presented in terms of the Brønsted-Lowry notion of acids and bases, as opposed to the Lewis acid-base theory (see acid-base reaction theories).

Preparation of buffer solutions

There are a couple of ways to prepare a buffer solution of a specific pH. In the first method, prepare a solution with an acid and its conjugate base by dissolving the acid form of the buffer in about 60% of the volume of water required to obtain the final solution volume. Then, measure the pH of the solution using a pH probe. The pH can be adjusted up to the desired value using a strong base like NaOH. If the buffer is made with a base and its conjugate acid, the pH can be adjusted using a strong acid like HCl. Once the pH is correct, dilute the solution to the final desired volume.

Alternatively, you can prepare solutions of both the acid form and base form of the solution. Both solutions must contain the same buffer concentration as the concentration of the buffer in the final solution. To get the final buffer, add one solution to the other while monitoring the pH.

In a third method, you can determine the exact amount of acid and conjugate base needed to make a buffer of a certain pH, using the Henderson-Hasselbach equation:

pH=pKa+log([A−][HA])

To prepare a buffer solution  with a specific pH , we first choose a weak acid  whose pka is close to the desired pH , then  the pKa and the pH values are substituted to obtain the (conjugate base)  /(acid ) ratio .these ratio can then be converted to molar quantities  for the preparation of the buffer solution

  • pH  =pka +log (conjugate base)
  •                                (acid)
  • Log (conjugate base) =0
  •                  (acid)
  • pH =pKa

APPLICATION OF BUFFER SOLUTIONS Their resistance to changes in pH makes buffer solutions very useful for chemical manufacturing and essential for many biochemical processes. The ideal buffer  for a particular pH has a pKa equal to the pH desired, since a solution of this buffer  would contain equal amounts of acid and base and be in the middle of the range of buffering capacity.

Buffer  are necessary to keep the correct pH for enzymes in many organisms to work. Many enzymes work only under very precise conditions; if the pH strays too far out of the margin, the enzymes slow or stop working and can denature, thus permanently disabling its catalytic activity.

A buffer of carbonic acid (H2CO3) and bicarbonate (HCO3) is present in blood plasma, to maintain a pH between 7.35 and 7.45.

Industrially, buffer solutions are used in fermentation processes and in setting the correct conditions for dyes used in colouring fabrics. They are also used in chemical analysis and calibration of pH meter

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