Course Content
Microscopes and Microscopy
MICROSCOPES AND MICROSCOPY OBJECTIVES By the end of this topic, the trainee should be able to: 1.Name various types of microscopes. 2.State the function of parts of a microscope. 3.Describe the use of compound light microscopes describe care and maintenance of compound microscopes. 4.Describe preparation of microscope slides
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The Cell
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define and explain meaning of terms. 2.State types of cells. 3.Describe the cell structure under the light microscope. 4.State the functions of cell organelles. 5.Describe the process of mitosis and meiosis. 6.Describe physiological processes of cells. 7.describe the techniques of cell isolation. 8.Describe the procedure of temporary cell preparation.
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Microorganisms
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Classify the major groups of microorganisms. 2.State the general characteristics of each group. 3.Explain their mode of nutrition and reproduction. 4.Describe culture media. 5.Describe culturing techniques for bacteria. 6.Describe methods for determining bacteria population. 7.Describe sterilization and disinfection techniques.
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Immunological Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Define terms. 2.Describe types of immunity. 3.Describe types of immune cells. 4.Describe the lymphoid organs and tissues. 5.Describe serological and immunological techniques.
0/8
Herbarium Techniques
OBJECTIVES By the end of this topic , the trainee should be able to: 1.Explain terms 2.Describe importance of collecting and preserving herbarium specimens 3.Describe sources of herbarium specimens 4.Describe collection of herbarium specimens 5.Describe preservation of herbarium specimens 6.Describe display of herbarium specimens
0/8
Museum Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of collecting and preserving museum specimens. 3.Describe sources of museum specimens. 4.Describe collection of museum specimens. 5.Describe preservation of museum specimens. 6.Describe display of museum specimens
0/5
Vivarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of vivarium. 3.Describe essential features of a vivarium. 4.Describe construction of a vivarium. 5.Describe maintenance of a vivarium.
0/4
Aquarium Techniques
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Explain terms. 2.Describe importance of aquariums. 3.Describe essential features of an aquarium tank. 4.Describe construction of an aquarium tank. 5.Describe maintenance of an aquarium tank.
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Laboratory Animals
OBJECTIVES The objective of this chapter is to give a better understanding of the technical requirements regarding handling, care and maintained of various laboratory animals In this chapter, we will; 1. Identify the various types of laboratory animals. 2.Discuss the general care and handling of laboratory animals. 3. Describe the various methods of restraining and humane killing laboratory animals 4.Discuss care of specific disease free (SPF)and Gnotobiotic animals
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Introduction to Ecology
OBJECTIVE By the end of this module, the trainee should be able to: 1.Explain terms. 2.Describe biotic and abiotic factors. 3.Explain adaptation of organisms to terrestrial and aquatic environment. 4.Describe the energy flow in ecosystem. 5.Explain estimation of population in ecosystem. 6.Describe influence of human activities on environment. 7.Describe basic biogeochemical cycles.
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Plant Anatomy and Physiology
OBJECTIVES By the end of this topic, the trainee should be able to: 1.Describe of plant parts and tissues. 2.Describe functions of various plant tissues. 3.Describe processes in plants .
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Biology Techniques For Science Laboratory Technicians
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Reproduction In Bacteria 

Reproduction in bacteria can occur through several mechanisms, the most common of which are binary fission and conjugation. Let’s explore each of these processes in more detail:

  1. Binary Fission: Binary fission is the primary mode of reproduction in bacteria. It is an asexual process where a single bacterium divides into two identical daughter cells. The steps involved in binary fission are as follows:

    a. DNA replication: The bacterial chromosome, a circular DNA molecule, undergoes replication to produce two identical copies.

    b. Elongation: The cell elongates, and the replicated DNA molecules move towards opposite ends of the bacterium.

    c. Septum formation: A septum, or a cell wall, forms at the midpoint of the bacterium, dividing it into two compartments.

    d. Division: The septum fully develops, leading to the physical separation of the two daughter cells.

    e. Cell separation: The daughter cells separate completely, and each can continue to grow and divide independently.

  2. Conjugation: Conjugation is a mechanism of genetic exchange between bacteria. It involves the transfer of genetic material, often in the form of plasmids, from a donor bacterium (F+ or male) to a recipient bacterium (F- or female). The steps involved in conjugation are as follows:

    a. Contact and bridge formation: The donor bacterium establishes contact with the recipient bacterium using specialized protein structures called pili. The pili form a bridge between the two cells.

    b. DNA transfer: The donor bacterium transfers a copy of its plasmid or part of its chromosomal DNA to the recipient through the pili bridge.

    c. Incorporation: The transferred DNA is incorporated into the recipient bacterium’s genome, allowing it to acquire new genetic traits.

    d. Replication: Both the donor and recipient bacterium replicate their DNA, ensuring that the genetic material is passed on to future generations.

  3. Other mechanisms: Besides binary fission and conjugation, bacteria can also reproduce through other mechanisms such as transformation and transduction, which involve the uptake of DNA from the environment or the transfer of DNA via bacteriophages (viruses that infect bacteria), respectively. However, these mechanisms are less common than binary fission and conjugation.

 While  most bacteria primarily reproduce asexually, some bacteria can also undergo sexual reproduction by forming specialized structures called endospores or by other variations in their reproductive processes. These variations contribute to the vast genetic diversity observed in bacterial populations.


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